%0 Generic %A Rossenkhan, Raabya %A Novitsky, Vladimir %A K. Sebunya, Theresa %A Musonda, Rosemary %A A. Gashe, Berhanu %A Essex, M. %D 2012 %T Viral Diversity and Diversification of Major Non-Structural Genes vif, vpr, vpu, tat exon 1 and rev exon 1 during Primary HIV-1 Subtype C Infection %U https://plos.figshare.com/articles/dataset/Viral_Diversity_and_Diversification_of_Major_Non_Structural_Genes_vif_vpr_vpu_tat_exon_1_and_rev_exon_1_during_Primary_HIV_1_Subtype_C_Infection/125279 %R 10.1371/journal.pone.0035491 %2 https://ndownloader.figshare.com/files/330541 %2 https://ndownloader.figshare.com/files/330596 %2 https://ndownloader.figshare.com/files/330643 %2 https://ndownloader.figshare.com/files/330685 %K viral %K diversification %K non-structural %K genes %K exon %K hiv-1 %K subtype %X

To assess the level of intra-patient diversity and evolution of HIV-1C non-structural genes in primary infection, viral quasispecies obtained by single genome amplification (SGA) at multiple sampling timepoints up to 500 days post-seroconversion (p/s) were analyzed. The mean intra-patient diversity was 0.11% (95% CI; 0.02 to 0.20) for vif, 0.23% (95% CI; 0.08 to 0.38) for vpr, 0.35% (95% CI; −0.05 to 0.75) for vpu, 0.18% (95% CI; 0.01 to 0.35) for tat exon 1 and 0.30% (95% CI; 0.02 to 0.58) for rev exon 1 during the time period 0 to 90 days p/s. The intra-patient diversity increased gradually in all non-structural genes over the first year of HIV-1 infection, which was evident from the vif mean intra-patient diversity of 0.46% (95% CI; 0.28 to 0.64), vpr 0.44% (95% CI; 0.24 to 0.64), vpu 0.84% (95% CI; 0.55 to 1.13), tat exon 1 0.35% (95% CI; 0.14 to 0.56 ) and rev exon 1 0.42% (95% CI; 0.18 to 0.66) during the time period of 181 to 500 days p/s. There was a statistically significant increase in viral diversity for vif (p = 0.013) and vpu (p = 0.002). No associations between levels of viral diversity within the non-structural genes and HIV-1 RNA load during primary infection were found. The study details the dynamics of the non-structural viral genes during the early stages of HIV-1C infection.

%I PLOS ONE