%0 Figure %A Peredo, Joan %A Villacé, Patricia %A Ortín, Juan %A de Lucas, Susana %D 2014 %T Association of miR-124 to hStau1 complexes in undifferentiated and differentiated neuroblastoma cells. %U https://plos.figshare.com/articles/figure/_Association_of_miR_124_to_hStau1_complexes_in_undifferentiated_and_differentiated_neuroblastoma_cells_/1251672 %R 10.1371/journal.pone.0113704.g005 %2 https://ndownloader.figshare.com/files/1806783 %K Staufen 1 mRNA targets %K dendritic arborisation %K Staufen 1. %K neuroblastoma cells %K translation repression %K Staufen 1 complexes %K neuroblastoma cell differentiation %K Staufen 1 %K Motor proteins %K localised translation %K Human Staufen 1 Associates %K intracellular localization %K Neuronal Cell Differentiation %K cell fate %X

Cultures of SH-SY5Y neuroblastoma cells were differentiated as described in Materials and Methods. Total cell extracts were isolated from cells prior to differentiation (day 0) or at a final stage of differentiation (day 10) and fractionated on a Sephacryl S-400 column. (A) The various fractions were analysed by Western-blot with antibodies specific for Ago2 and hStau1 The gels to analyse hStau1 were run longer than in Fig. 4 to better separate hStau1 55 and 63 kDa isoforms. (B) RNA was isolated from the fraction pools indicated and the concentration of miR-124 was determined by TaqMan RT-qPCR. (C) The amounts of hStau155 and hStau163 isoforms were determined by Western-blot (see A) and their ratios is presented as average and standard deviations of 3 determinations. * indicates a p-value<0.05 in a two-tailed Student's t-test.

%I PLOS ONE