10.1371/journal.pone.0113704.g004 Joan Peredo Joan Peredo Patricia Villacé Patricia Villacé Juan Ortín Juan Ortín Susana de Lucas Susana de Lucas Analysis of hStau1 complexes and the associated miRNAs in human neuroblastoma cells. Public Library of Science 2014 Staufen 1 mRNA targets dendritic arborisation Staufen 1. neuroblastoma cells translation repression Staufen 1 complexes neuroblastoma cell differentiation Staufen 1 Motor proteins localised translation Human Staufen 1 Associates intracellular localization Neuronal Cell Differentiation cell fate 2014-11-25 03:06:46 Figure https://plos.figshare.com/articles/figure/_Analysis_of_hStau1_complexes_and_the_associated_miRNAs_in_human_neuroblastoma_cells_/1251670 <p>Soluble extracts derived from the SH-SY5Y neuroblastoma cell line were filtered on a Sephacryl S-400 column. (A) The various fractions were analysed by Western-blot with antibodies specific for Ago2, RCK/p54 and hStau1. (B) The fractions were grouped in 4 pools: F1 (17, 18), F2 (21,22,23), F3 (26,27,28,29) and F4 (34,35,36 as a negative control). RNA was isolated in each pool and analysed by TaqMan RT-qPCR to quantify the six miRNAs most prevalent in hStau1 complexes. (C) The amounts of miR-124 and miR-9 were determined in the fractions pools F1 to F4 derived from 3 independent filtration experiments. Values are averages and standard deviations and represent the amount of miRNA present in each fraction pool as percentage of total miRNA recovered from F1+F2+F3+F4 pools.</p>