10.1371/journal.pone.0113704.g004
Joan Peredo
Joan
Peredo
Patricia Villacé
Patricia
Villacé
Juan Ortín
Juan
Ortín
Susana de Lucas
Susana
de Lucas
Analysis of hStau1 complexes and the associated miRNAs in human neuroblastoma cells.
Public Library of Science
2014
Staufen 1 mRNA targets
dendritic arborisation
Staufen 1.
neuroblastoma cells
translation repression
Staufen 1 complexes
neuroblastoma cell differentiation
Staufen 1
Motor proteins
localised translation
Human Staufen 1 Associates
intracellular localization
Neuronal Cell Differentiation
cell fate
2014-11-25 03:06:46
Figure
https://plos.figshare.com/articles/figure/_Analysis_of_hStau1_complexes_and_the_associated_miRNAs_in_human_neuroblastoma_cells_/1251670
<p>Soluble extracts derived from the SH-SY5Y neuroblastoma cell line were filtered on a Sephacryl S-400 column. (A) The various fractions were analysed by Western-blot with antibodies specific for Ago2, RCK/p54 and hStau1. (B) The fractions were grouped in 4 pools: F1 (17, 18), F2 (21,22,23), F3 (26,27,28,29) and F4 (34,35,36 as a negative control). RNA was isolated in each pool and analysed by TaqMan RT-qPCR to quantify the six miRNAs most prevalent in hStau1 complexes. (C) The amounts of miR-124 and miR-9 were determined in the fractions pools F1 to F4 derived from 3 independent filtration experiments. Values are averages and standard deviations and represent the amount of miRNA present in each fraction pool as percentage of total miRNA recovered from F1+F2+F3+F4 pools.</p>