%0 Figure %A Nandakumar, Subhadra %A Kannanganat, Sunil %A E. Posey, James %A Rao Amara, Rama %A B. Sable, Suraj %D 2014 %T BCG vaccination induces polyfunctional CD4+ but monofunctional CD8+ T-cell response. %U https://plos.figshare.com/articles/figure/_BCG_vaccination_induces_polyfunctional_CD4_but_monofunctional_CD8_T_cell_response_/1250239 %R 10.1371/journal.pone.0113951.g002 %2 https://ndownloader.figshare.com/files/1805090 %K novel vaccination strategies %K CTLA %K Inhibitory Markers Correspond %K ifn %K Simultaneous Upregulation %K cytokine polyfunctionality %K protection %K contraction phase %K Mycobacterium tuberculosis %K BCG persistence %K tuberculosis infection %K response kinetics %K cytokine production %K results question %K CD 62L memory marker expression %K tnf %K il %K parenteral BCG vaccination %K KLRG %K attenuated vaccine %X

(A–C) The lung and spleen cells of i.n. and s.c. BCG-vaccinated mice (n = 4/time point/group) were stimulated with WCL, and the magnitudes and polyfunctionality of CD3+CD4+ and CD3+CD8+ T cells in-terms of IFN-γ, IL-2 and TNF-α production were determined using polychromatic flow cytometry. (A) The longitudinal changes in the magnitudes of WCL-specific individual IFN-γ, IL-2 or TNF-α-producing cells among CD4+ or CD8+ T cells in the lung and spleen are plotted. (B) Representative dot plots show the frequency of WCL-specific IFN-γ and IL-2 or IFN-γ and TNF-α-producing cells among splenic CD4+ and CD8+ T cells from one mouse per vaccinated group at week 32 in comparison with age-matched naïve control, and the distributions of single, double and triple-cytokine-producers are illustrated. (C) The magnitudes of 7 possible combinations of WCL-specific cytokine-producing subsets constituting total cytokine+ CD4+ or CD8+ T cells in the lung, spleen, cervical lymph node (CLN) and inguinal lymph node (ILN) are depicted. The data (A, C) are mean ± s.e.m. responses of 2 (at week 3, 78 and 104), 3 (at week 32) or 4 (at week 6 and 12) independent experiments.

%I PLOS ONE