10.1371/journal.pone.0113951.g001
Subhadra Nandakumar
Subhadra
Nandakumar
Sunil Kannanganat
Sunil
Kannanganat
James E. Posey
James
E. Posey
Rama Rao Amara
Rama
Rao Amara
Suraj B. Sable
Suraj
B. Sable
BCG persists and induces a durable cell-mediated immunity in mice.
Public Library of Science
2014
novel vaccination strategies
CTLA
Inhibitory Markers Correspond
ifn
Simultaneous Upregulation
cytokine polyfunctionality
protection
contraction phase
Mycobacterium tuberculosis
BCG persistence
tuberculosis infection
response kinetics
cytokine production
results question
CD 62L memory marker expression
tnf
il
parenteral BCG vaccination
KLRG
attenuated vaccine
2014-11-24 02:46:45
Figure
https://plos.figshare.com/articles/figure/_BCG_persists_and_induces_a_durable_cell_mediated_immunity_in_mice_/1250238
<p>(A–B) BALB/c mice were vaccinated with 1×10<sup>6</sup> CFU of BCG Copenhagen by intranasal (i.n.) or subcutaneous (s.c.) route. At 7 different time points after vaccination at the same time, mice were euthanized and their lungs and spleen were isolated. (A) BCG load in the two organs of individual mice was determined. Detection limit of the assay as shown by dotted line was 30 CFU/organ, and data shown are mean CFU ± s.e.m. in the lung (left) and spleen (right) from two independent experiments using 5 individually analyzed mice per group at each time point. The experiment was not performed at the104-week time point. (*<i>P</i><0.05, ** <i>P</i><0.01, *** <i>P</i><0.001, **** <i>P</i><0.0001 using 1-way analysis of variance (ANOVA) with Tukey's post-test). (B) In parallel, pooled cells from the lung (left) or spleen (right) of BCG-vaccinated and naïve mice (<i>n</i> = 4/time point/group) were stimulated with WCL in a cultured ELISPOT assay and IFN-γ, IL-4 and IL-17A SFU were enumerated. Data are means ± s.e.m. of individual cytokine SFU constituting the total cytokine responses of 2 (at week 3, 78 and 104), 3 (at week 32) or 4 (at week 6 and 12) independent experiments evaluated in triplicate. Responses are significant (<i>P</i><0.001) in vaccinated compared to naïve mice at 6 time points after week 3 using 1-way ANOVA with Tukey's post-test.</p>