%0 Journal Article %A Kakui, Yasutaka %A Uhlmann, Frank %D 2020 %T Efficient depletion of fission yeast condensin by combined transcriptional repression and auxin-induced degradation. %U https://crick.figshare.com/articles/journal_contribution/Efficient_depletion_of_fission_yeast_condensin_by_combined_transcriptional_repression_and_auxin-induced_degradation_/12490310 %2 https://ndownloader.figshare.com/files/23168246 %K Auxin-inducible degron %K Chromosome condensation %K Condensin %K Fission yeast %K SMC complex %K Transcriptional repression %K Adenosine Triphosphatases %K Cell Cycle Proteins %K Chromatin %K Chromosomes, Fungal %K DNA-Binding Proteins %K Indoleacetic Acids %K Interphase %K Mitosis %K Multiprotein Complexes %K Proteolysis %K Transcription, Genetic %K Yeasts %K Uhlmann FC001198 %K 0601 Biochemistry and Cell Biology %K 0399 Other Chemical Sciences %K Developmental Biology %X Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts chromatin to form condensed chromosomes in mitosis. Complete condensin inactivation is necessary to reveal how condensin converts interphase chromatin into mitotic chromosomes. Here, we have developed a condensin depletion system in fission yeast that combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient condensin depletion without need for a temperature shift. Our system is useful when studying how condensin contributes to chromosome architecture and is applicable to the study of other SMC complexes. %I The Francis Crick Institute