%0 Generic %A Basson, Etienne %A Zeiler, Gareth %A Kamerman, Peter %A Meyer, Leith %D 2020 %T Analysis scripts and supplementary files: Blood colour can be used to assess arterial blood oxygenation in immobilised impala (Aepyceros melampus) %U https://figshare.com/articles/dataset/Analysis_scripts_and_supplementary_files_Blood_colour_can_be_used_to_assess_arterial_blood_oxygenation_in_immobilised_impala_Aepyceros_melampus_/12403112 %R 10.6084/m9.figshare.12403112.v1 %2 https://ndownloader.figshare.com/files/22850717 %K Fractional oxyhaemoglobin saturation %K CIE L*a*b* %K Functional oxyhaemoglobin saturation %K Veterinary Medicine %X
Background To determine the relationship between arterial blood colour (CIE L*a*b*) and blood oxygenation [functional oxyhaemoglobin saturation (SaO2) and fractional oxyhaemoglobin saturation (FO2Hb)], and if arterial blood colour can be used to predict arterial blood oxygenation.

Methods A prospective randomised crossover study where 11 female impala were immobilised with potent opioids. Arterial blood was collected anaerobically into heparinised syringes and analysed immediately using spectrocolourimetry and co-oximetry. Data were analysed by modelling the relationship between predicted CIE L*a*b* components and SaO2 and FO2Hb, and assessing the subjective ordering of the resulting colour palette by 20 observers using a modified version of the Farnsworth-Munsell hue test.

Results The second-order polynomial (quadratic) model produced the best fit for all three CIE L*a*b* components for SaO2 and FO2Hb. The regression models were used to generate predicted CIE L*a*b* co-ordinates over a range of SaO2 and FO2Hb percentages (15 to 95%). The resulting colour palettes were correctly ordered by all observers, in the SaO2 and FO2Hb ranges of 45 to 95% saturation.

Conclusions An association between the blood colour components (CIE L*a*b*) and SaO2 and FO2Hb exists and arterial blood colour can be used to reliably estimate arterial blood oxygenation of impala.
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