10.1371/journal.pone.0111550.g003 Alessandra Fazzini Alessandra Fazzini Vanessa D’Antongiovanni Vanessa D’Antongiovanni Laura Giusti Laura Giusti Ylenia Da Valle Ylenia Da Valle Federica Ciregia Federica Ciregia Ilaria Piano Ilaria Piano Antonella Caputo Antonella Caputo Anna Maria D’Ursi Anna Maria D’Ursi Claudia Gargini Claudia Gargini Antonio Lucacchini Antonio Lucacchini Maria Rosa Mazzoni Maria Rosa Mazzoni NCI-H28 and REN cells express significant less amount of PAR<sub>1</sub> on plasma membrane. Public Library of Science 2014 plasma membrane localization mesothelioma cell lines dysfunctional PAR 1 Malignant Pleural Mesothelioma Cell Line mesothelioma REN cells PAR 1 cell surface PAR 1 expression 2014-11-03 03:53:37 Figure https://plos.figshare.com/articles/figure/_NCI_H28_and_REN_cells_express_significant_less_amount_of_PAR_1_on_plasma_membrane_/1227675 <p>Cell surface PAR<sub>1</sub> expression was measured by ELISA using a polyclonal antibody which recognizes the N-terminus of PAR<sub>1</sub><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111550#pone.0111550-Paing1" target="_blank">[31]</a>. Antibody binding to fixed cells was detected by horseradish peroxidise-conjugated secondary antibody. Data represent the mean ± SEM of three independent experiments performed in triplicate. The differences in cell surface PAR<sub>1</sub> expression between Ctrl (Met-5A cells) and MPM (NCI-H28 and REN) cellswere significant (***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test.</p>