10.1371/journal.pone.0111550.g003
Alessandra Fazzini
Alessandra
Fazzini
Vanessa D’Antongiovanni
Vanessa
D’Antongiovanni
Laura Giusti
Laura
Giusti
Ylenia Da Valle
Ylenia
Da Valle
Federica Ciregia
Federica
Ciregia
Ilaria Piano
Ilaria
Piano
Antonella Caputo
Antonella
Caputo
Anna Maria D’Ursi
Anna Maria
D’Ursi
Claudia Gargini
Claudia
Gargini
Antonio Lucacchini
Antonio
Lucacchini
Maria Rosa Mazzoni
Maria
Rosa Mazzoni
NCI-H28 and REN cells express significant less amount of PAR<sub>1</sub> on plasma membrane.
Public Library of Science
2014
plasma membrane localization
mesothelioma cell lines
dysfunctional PAR 1
Malignant Pleural Mesothelioma Cell Line
mesothelioma REN cells
PAR 1
cell surface PAR 1 expression
2014-11-03 03:53:37
Figure
https://plos.figshare.com/articles/figure/_NCI_H28_and_REN_cells_express_significant_less_amount_of_PAR_1_on_plasma_membrane_/1227675
<p>Cell surface PAR<sub>1</sub> expression was measured by ELISA using a polyclonal antibody which recognizes the N-terminus of PAR<sub>1</sub><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111550#pone.0111550-Paing1" target="_blank">[31]</a>. Antibody binding to fixed cells was detected by horseradish peroxidise-conjugated secondary antibody. Data represent the mean ± SEM of three independent experiments performed in triplicate. The differences in cell surface PAR<sub>1</sub> expression between Ctrl (Met-5A cells) and MPM (NCI-H28 and REN) cellswere significant (***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test.</p>