%0 Figure %A Cloonan, Nicole %A Cloonan, Nicole %D 2014 %T Experimental methods of miRNA target detection. %U https://auckland.figshare.com/articles/figure/Experimental_methods_of_miRNA_target_detection_/1222672 %R 10.6084/m9.figshare.1222672.v1 %2 https://ndownloader.figshare.com/files/3242615 %K mirnas %K miRNA biogenesis %K miRNA binding sites %K Molecular Biology %K Biological Techniques %K Genetics %X

This figure illustrates the six major methods of experimentally determining miRNA binding sites in mRNAs: (a) genome editing of predicted binding sites; (b) reporter gene assays; (c) gene-expression after miRNA modulation; (d) degradome sequencing; (e) chromatin-linked immuno-precipitation; and (f)

biotin-linked chromatography.

%I The University of Auckland