10.1371/journal.pgen.1004718.g004 Peter Bou Dib Peter Bou Dib Bettina Gnägi Bettina Gnägi Fiona Daly Fiona Daly Virginie Sabado Virginie Sabado Damla Tas Damla Tas Dominique A. Glauser Dominique A. Glauser Peter Meister Peter Meister Emi Nagoshi Emi Nagoshi Partial loss of <i>Fer2</i> expression impairs the viability of PAM neurons in aging flies. Public Library of Science 2014 p 48 Homologs DA neurons PAM neuron degeneration p 48 homologs share neurodegenerative movement disorder Drosophila p 48 homolog pd ros Fer 2 expression DA neuron survival Reactive oxygen species Protecting Dopaminergic Neurons oxidative stress oxidative stress challenge upregulates Fer 2 expression Oxidative Stress Parkinson 2014-10-23 17:16:41 Figure https://plos.figshare.com/articles/figure/_Partial_loss_of_Fer2_expression_impairs_the_viability_of_PAM_neurons_in_aging_flies_/1220341 <p>Quantification of PAM neurons in the flies of different genotypes detected by anti-TH staining at indicated ages. Box boundaries are the 25th and 75th percentiles, the horizontal line across the box is the median, and the whiskers are the lowest and highest values that are not outliers. The open circles represent outliers and closed circles are extreme cases. *p<0.05, **p<0.01, *** p<0.001, comparing <i>Fer2<sup>2</sup>/+</i> and <i>Fer2<sup>2</sup></i> at the same age (A), or between <i>miR-Fer2-N</i> and <i>miR-Fer2-4/</i>-<i>5</i> at the same age (B-D). Sample sizes are indicated in the <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004718#s4" target="_blank">Materials and Methods</a>. (A) PAM neurons in <i>Fer2<sup>2</sup></i> and the control (<i>Fer2<sup>2</sup>/+</i>). (B) Constitutive expression of <i>miR Fer2s</i> by <i>Fer2-GAL4.</i> (C) Constitutive expression of <i>miR Fer2s</i> by <i>R58E02-GAL4</i>. (D) Adult-specific <i>Fer2</i> knockdown and the control. <i>Fer2-GAL4</i>, <i>tub-gal80<sup>ts</sup></i>: control without UAS-transgene.</p>