10.1371/journal.pone.0110911.g006 Weiwei Li Weiwei Li Gang Wang Gang Wang Wulong Liang Wulong Liang Kai Kang Kai Kang Kangkang Guo Kangkang Guo Yanming Zhang Yanming Zhang Evaluation of shRNA transfection-efficiency and integrin β3 deficiency. Public Library of Science 2014 iec ICDC porcine kidney epithelial CSFV proliferation rate pcr CSFV infection CSFV proliferation ifc pk ec integrin st Fever Virus Classical swine umbilical vein 72 h post CSFV infection icc swine testicles epithelial 2014-10-23 03:27:28 Figure https://plos.figshare.com/articles/figure/_Evaluation_of_shRNA_transfection_efficiency_and_integrin_946_3_deficiency_/1213578 <p><b>A)</b> Fluorescence pictures of cells with shRNAs transiently transfected for 24 h. 976, 1110 and 1755 denote the targeting positions on integrin β3 mRNA sequence. nc denotes the negative-control construct which generates non-specific interfere RNA. <b>B)</b> Fluorescence pictures of cells transiently transfected with shRNA976, shRNA1110, shRNA1755 or nc for 48 h. <b>C)</b> Quantitative analysis of fluorescence intensity in Fig. 6A and 6B evaluated by ImageJ. <b>D)</b> Assessment of integrin β3 deficiency at 48 h post shRNA transiently transfection using two-step qPCR. ***p<0.001 denotes extremely significant difference. n.s. denotes no significant difference.</p>