10.1371/journal.pone.0110911.g006
Weiwei Li
Weiwei
Li
Gang Wang
Gang
Wang
Wulong Liang
Wulong
Liang
Kai Kang
Kai
Kang
Kangkang Guo
Kangkang
Guo
Yanming Zhang
Yanming
Zhang
Evaluation of shRNA transfection-efficiency and integrin β3 deficiency.
Public Library of Science
2014
iec
ICDC
porcine kidney epithelial
CSFV proliferation rate
pcr
CSFV infection
CSFV proliferation
ifc
pk
ec
integrin
st
Fever Virus Classical
swine umbilical vein
72 h post CSFV infection
icc
swine testicles epithelial
2014-10-23 03:27:28
Figure
https://plos.figshare.com/articles/figure/_Evaluation_of_shRNA_transfection_efficiency_and_integrin_946_3_deficiency_/1213578
<p><b>A)</b> Fluorescence pictures of cells with shRNAs transiently transfected for 24 h. 976, 1110 and 1755 denote the targeting positions on integrin β3 mRNA sequence. nc denotes the negative-control construct which generates non-specific interfere RNA. <b>B)</b> Fluorescence pictures of cells transiently transfected with shRNA976, shRNA1110, shRNA1755 or nc for 48 h. <b>C)</b> Quantitative analysis of fluorescence intensity in Fig. 6A and 6B evaluated by ImageJ. <b>D)</b> Assessment of integrin β3 deficiency at 48 h post shRNA transiently transfection using two-step qPCR. ***p<0.001 denotes extremely significant difference. n.s. denotes no significant difference.</p>