Calvenzani, Valentina Testoni, Barbara Gusmaroli, Giuliana Lorenzo, Mariangela Gnesutta, Nerina Petroni, Katia Mantovani, Roberto Tonelli, Chiara Figure S1 - Interactions and CCAAT-Binding of <em>Arabidopsis thaliana</em> NF-Y Subunits <p><b>TnT and recombinant proteins production.</b> <b>A.</b> AtNF-YA, AtNF-YB and AtNF-YC subunits were synthesized and <sup>35</sup>S-labeled by coupled transcription and translation in nuclease-treated rabbit reticulocyte lysate (TnT, Promega). <b>B.</b> His-tagged AtNF-YA6, AtNF-YB2 and AtNF-YB6, AtNF-YC3 and AtNF-YC7 have been produced in <i>E. Col</i>i and purified by Nichel-Agarose columns (Sigma). Load (L), flow-through (FT), wash (W) and eluted (E) fractions of NTA Nickel columns are shown.</p> <p>(TIF)</p> interactions;ccaat-binding;nf-y;subunits 2012-08-17
    https://plos.figshare.com/articles/figure/Interactions_and_CCAAT_Binding_of_Arabidopsis_thaliana_NF_Y_Subunits/121054
10.1371/journal.pone.0042902.s001