Figure S1 - Interactions and CCAAT-Binding of <em>Arabidopsis thaliana</em> NF-Y Subunits Valentina Calvenzani Barbara Testoni Giuliana Gusmaroli Mariangela Lorenzo Nerina Gnesutta Katia Petroni Roberto Mantovani Chiara Tonelli 10.1371/journal.pone.0042902.s001 https://plos.figshare.com/articles/figure/Interactions_and_CCAAT_Binding_of_Arabidopsis_thaliana_NF_Y_Subunits/121054 <p><b>TnT and recombinant proteins production.</b> <b>A.</b> AtNF-YA, AtNF-YB and AtNF-YC subunits were synthesized and <sup>35</sup>S-labeled by coupled transcription and translation in nuclease-treated rabbit reticulocyte lysate (TnT, Promega). <b>B.</b> His-tagged AtNF-YA6, AtNF-YB2 and AtNF-YB6, AtNF-YC3 and AtNF-YC7 have been produced in <i>E. Col</i>i and purified by Nichel-Agarose columns (Sigma). Load (L), flow-through (FT), wash (W) and eluted (E) fractions of NTA Nickel columns are shown.</p> <p>(TIF)</p> 2012-08-17 00:17:34 interactions ccaat-binding nf-y subunits