da Costa Pereira, Gizele Lucia Almeida, Tamires Cunha Seibert, Janaina Brandão Amparo, Tatiane Roquete de Oliveira Aguiar Soares, Rodrigo Dian Rodrigues, Ivanildes Vasconcelos de Souza, Gustavo Henrique Bianco Santos, Orlando David Henrique dos da Silva, Glenda Nicioli Antitumor effect of <i>Cymbopogon densiflorus</i> (Linneu) essential oil in bladder cancer cells <p>The aim of this study was to analyse the antitumor effect of the <i>Cymbopogon densiflorus</i> essential oil <i>in silico</i> and <i>in vitro</i> on bladder cancer cells RT4 and T24, with different <i>TP53</i> status. The oil was extracted by hydrodistillation and the gas chromatography coupled to the mass spectrometry was used for characterisation. <i>In silico</i> analysis was carried out by Pass online software. Cytotoxicity, cell proliferation, cell cycle progression, apoptosis and wound healing assays were performed. Five major compounds were identified. <i>In silico</i> analysis showed that major compounds present high potential for antitumor activities. The treatment with <i>C. densiflorus</i> essential oil reduced cell viability of bladder cancer cells. Only in wild-type cells, the increase of apoptosis rates and the decrease of cell migration were observed. In conclusion, the <i>C. densiflorus</i> essential oil presents antitumor effects on <i>TP53</i> wild-type and mutated bladder cancer cells, however, the mechanism of action is <i>TP53</i> status-dependent.</p> Bladder cancer;Cymbopogon densiflorus;cytotoxicity;essential oil;in silico analyses 2020-04-03
    https://tandf.figshare.com/articles/journal_contribution/Antitumor_effect_of_i_Cymbopogon_densiflorus_i_Linneu_essential_oil_in_bladder_cancer_cells/12073218
10.6084/m9.figshare.12073218.v1