<i>In vivo</i> analysis of thrombus formation in arthritic mice Shoko Tsuji Tomoyuki Mukai Hiroyasu Hirano Yoshitaka Morita 10.6084/m9.figshare.12046299.v1 https://tandf.figshare.com/articles/media/_i_In_vivo_i_analysis_of_thrombus_formation_in_arthritic_mice/12046299 <p>Rheumatoid arthritis (RA) is characterized by inflammation in multiple joints. In addition to causing joint destruction, the persistent systemic inflammation with RA increases the risk of cardiovascular disease. Although there are <i>in vitro</i> studies showing the prothrombotic effect of inflammatory cytokines, especially TNF, <i>in vivo</i> experimental evidence is lacking due to the complexity of <i>in vivo</i> modeling and observation. In this study, we aimed to model <i>in vivo</i> thrombus formation in arthritic mice and to determine whether the arthritic condition would further promote thrombotic formation.</p> <p>Human TNF-transgenic mice were used as the arthritis model. Thrombus formation was observed on the testicular arterioles. Thrombus formation was induced by reactive oxygen species generated from hematoporphyrin under laser irradiation.</p> <p>Platelet thrombus formation was observed in real-time using a laser confocal microscopy in both wild-type and arthritic mice. Quantitative analyses revealed that no significant differences were observed in thrombus formation, represented by platelet attachment time and vascular obstruction time, in our experimental setting.</p> <p>Although we confirmed the usefulness of this novel technique for <i>in vivo</i> studies, further investigation is required to conclude the possible mechanism of prothrombotic phenotypes under inflammatory conditions.</p> 2020-03-30 09:14:24 Arthritis in vivo analysis inflammation platelet thrombosis