%0 Figure %A Lunelli, Michele %A Kamprad, Antje %A Bürger, Jörg %A Mielke, Thorsten %A Spahn, Christian M. T. %A Kolbe, Michael %D 2020 %T Architecture of the outer regions of the basal body. %U https://plos.figshare.com/articles/figure/Architecture_of_the_outer_regions_of_the_basal_body_/11893476 %R 10.1371/journal.ppat.1008263.g002 %2 https://ndownloader.figshare.com/files/21812940 %K syringe-shaped protein translocation nanomachine %K High-resolution structures %K secretin oligomer %K export apparatus core %K IM subunits bind %K cyclic symmetry %K β- sheet augmentation %K periplasmic N-terminal connector %K 3.5 MDa %K helical architecture %K cryo-electron microscopy structure %K secretin connector %K Type III Secretion Systems %K effector translocation %K membrane ring %K Cryo-EM structure %K T 3SS needle %K extracellular needle %K infection stages %K T 3SS %K host cells %K effector proteins %K Shigella T 3SS needle %K basal body %K membrane-embedded basal body %K export apparatus cage %K cryo-EM map %K channel system %K Shigella type III needle %X

(A) Composite map of the highest-resolution maps obtained for the outer regions of the needle complex (left panel), including the focused IM ring map with C24 symmetry, the connector map from the focused IM-connector reconstruction with C8 symmetry and the OM ring map from C1 reconstruction. The cyclic symmetry of the three regions is indicated on the left. (B) Atomic models of the corresponding needle complex proteins built using maps depicted in (A): MxiG (blue), MxiJ (orange) and MxiD (green).

%I PLOS Pathogens