Needle complex reconstruction and symmetry analysis.
Michele Lunelli
Antje Kamprad
Jörg Bürger
Thorsten Mielke
Christian M. T. Spahn
Michael Kolbe
10.1371/journal.ppat.1008263.g001
https://plos.figshare.com/articles/figure/Needle_complex_reconstruction_and_symmetry_analysis_/11893473
<p>(<b>A</b>) Cross-section and side view of the symmetry-free (C1) reconstruction of the <i>Shigella</i> needle complex. The structural features and their dimensions are indicated. Arrows show the position of the slices shown in panel B. (<b>B</b>) Horizontal slices of the periplasmic IM ring (bottom panel),the connector (middle panel) and the OM ring (upper panel, from non-sharpened map). (<b>C</b>) Rotational autocorrelation analysis of the three slices shown in panel B indicating C24 symmetry for the IM ring (24 peaks spaced by 15 degrees), C16 symmetry for the connector (16 peaks spaced by 22.5 degrees) and C15 symmetry for the OM ring (15 peaks spaced by 24 degrees). A circular mask with inner radius 55 Å and outer radius 76 Å was applied to the OM ring slice to highlight the 15-fold periodicity.</p>
2020-02-24 18:49:09
syringe-shaped protein translocation nanomachine
High-resolution structures
secretin oligomer
export apparatus core
IM subunits bind
cyclic symmetry
β- sheet augmentation
periplasmic N-terminal connector
3.5 MDa
helical architecture
cryo-electron microscopy structure
secretin connector
Type III Secretion Systems
effector translocation
membrane ring
Cryo-EM structure
T 3SS needle
extracellular needle
infection stages
T 3SS
host cells
effector proteins
Shigella T 3SS needle
basal body
membrane-embedded basal body
export apparatus cage
cryo-EM map
channel system
Shigella type III needle