10.6084/m9.figshare.11855415.v3
Aneta Gandalovicova
Aneta
Gandalovicova
Quantitative analysis of cell migration
figshare
2020
cell migration
Cell Biology
2020-02-14 15:24:20
Figure
https://figshare.com/articles/figure/Quantification_of_cell_migration/11855415
Quantitative analysis of cell migration. HT1080-icaRhoA cells (a) and HT1080 cells (b) were<br>embedded in rat-tail collagen (1mg/ml) with or without GM6001. 12 hours later, migration of<br>cells of the mesenchymal phenotype (HT1080-icaRhoA control, HT1080 DMSO) and amoeboid<br>phenotype (HT1080-icaRhoA doxycycline, HT1080 dasatinib) was monitored by wide-field<br>microscopy for 15 hours. Three independent experiments were evaluated. Track plots were<br>generated using Chemotaxis Tool in ImageJ. For each condition, one representative track plot<br>is shown. Relative differences in the mean length of the tracks were analyzed with two-way<br>ANOVA (replicate + treatment group) followed by Tukey's HSD test. Statistically significant<br>differences are marked with the respective p-values.