%0 Figure %A van der Velden, Jos L. %A Alcorn, John F. %A Chapman, David G. %A Lundblad, Lennart K. A. %A Irvin, Charles G. %A Davis, Roger J. %A Butnor, Kelly %A Janssen-Heininger, Yvonne M. W. %D 2020 %T Conditional ablation of JNK1 from lung epithelial cells attenuates lung fibrosis induced by adTGFβ1. %U https://plos.figshare.com/articles/figure/Conditional_ablation_of_JNK1_from_lung_epithelial_cells_attenuates_lung_fibrosis_induced_by_adTGF_1_/11605932 %R 10.1371/journal.pone.0226904.g004 %2 https://ndownloader.figshare.com/files/21006006 %K Jnk 1 allele %K alveolar type II epithelial cells %K JNK 1 %K IPF %K type II pneumocytes %K mouse models %K Epithelial Jnk 1 ablation %K c-Jun-N-terminal kinase 1 %K CCSP-directed Cre recombinase-mediated ablation %K epithelial JNK 1 %K Jnk 1 form %K growth factor beta %K AdTGF β1-induced fibrosis %K tissue %K LoxP-flanked Jnk 1 alleles %K airway epithelia %K Jun-N-terminal kinase 1 attenuates %X

JNK1 was selectively ablated from the bronchiolar epithelial cells and type II cells using a CCSP promoter driving a reverse tetracycline activator (rtTA), the tetracyclin operon driving CRE recombinase (TetOP-Cre), and mice carrying a LoxP-flanked Jnk1 allele. Assessment of total collagen content in lung tissue 3 weeks following administration of bleomycin via hydroxyproline content (A), Masson’s trichrome staining (B), Sircol assay (C) and semi-quantitative evaluation of Masson’s trichrome reactive material (D). E: Assessment of pulmonary fibrosis, using the sircol assay in response to AdTGFβ1 in mice containing the CCSP-rtTA, TetO-Cre, and Jnk1 loxP/loxP alleles, or CCSP-rtTA, TetO-Cre alleles, in the absence of doxycycline-containing food. (WT- Jnk1: AdCtr n = 6, adTGFβ1 n = 8, ΔEpi Jnk1: AdCtr n = 5, adTGFβ1 n = 8 respectively mice/group from 2 independent experiments). * p< 0.05 compared to the adCtr control groups. p< 0.05 compared to respective WT groups. (ANOVA). Scale bars: 50 μm.

%I PLOS ONE