Depiction of the conditional ablation strategy of JNK1 from lung epithelial cells.
Jos L. van der Velden
John F. Alcorn
David G. Chapman
Lennart K. A. Lundblad
Charles G. Irvin
Roger J. Davis
Kelly Butnor
Yvonne M. W. Janssen-Heininger
10.1371/journal.pone.0226904.g002
https://plos.figshare.com/articles/figure/Depiction_of_the_conditional_ablation_strategy_of_JNK1_from_lung_epithelial_cells_/11605926
<p><b>(A</b>) Schematic of JNK1 ablation from airway epithelial cells using transgenic mice expressing CCSP-rtTA, TetO-Cre, and <i>Jnk1</i><sup>loxP/loxP</sup> alleles. Upon feeding doxycycline (dox)- containing food, JNK1 is ablated from airway epithelial cells, designated as ΔEpi <i>Jnk1</i>. (<b>B</b>) Confirmation of ablation of JNK1 in airway epithelial cells. 3 weeks after initiation of Dox feeding, single cells lung suspensions were created and cells were sorted by flow cytometry. The EpCAM positive, CD45 negative, Sca1 low fraction of lung cells was isolated for assessment of JNK1 expression via Western Blot analysis. Note that JNK1 was only ablated from the EpCAM positive, CD45 negative, Sca1low fraction of lung cells. (<b>C</b>) Visualization of Cre-recombination in CCSP-rtTA, TetO-Cre-expressing mice which were bred with Tomato red reporter mice. Red: Cre-expressing cells. Scale bars: 50 μm.</p>
2020-01-14 18:26:16
Jnk 1 allele
alveolar type II epithelial cells
JNK 1
IPF
type II pneumocytes
mouse models
Epithelial Jnk 1 ablation
c-Jun-N-terminal kinase 1
CCSP-directed Cre recombinase-mediated ablation
epithelial JNK 1
Jnk 1 form
growth factor beta
AdTGF β1-induced fibrosis
tissue
LoxP-flanked Jnk 1 alleles
airway epithelia
Jun-N-terminal kinase 1 attenuates