%0 Figure %A van der Velden, Jos L. %A Alcorn, John F. %A Chapman, David G. %A Lundblad, Lennart K. A. %A Irvin, Charles G. %A Davis, Roger J. %A Butnor, Kelly %A Janssen-Heininger, Yvonne M. W. %D 2020 %T Phospho-JNK immunoreactivity is increased in lung tissues in settings of airways and parenchymal fibrosis. %U https://plos.figshare.com/articles/figure/Phospho-JNK_immunoreactivity_is_increased_in_lung_tissues_in_settings_of_airways_and_parenchymal_fibrosis_/11605923 %R 10.1371/journal.pone.0226904.g001 %2 https://ndownloader.figshare.com/files/21005997 %K Jnk 1 allele %K alveolar type II epithelial cells %K JNK 1 %K IPF %K type II pneumocytes %K mouse models %K Epithelial Jnk 1 ablation %K c-Jun-N-terminal kinase 1 %K CCSP-directed Cre recombinase-mediated ablation %K epithelial JNK 1 %K Jnk 1 form %K growth factor beta %K AdTGF β1-induced fibrosis %K tissue %K LoxP-flanked Jnk 1 alleles %K airway epithelia %K Jun-N-terminal kinase 1 attenuates %X

(A) p-JNK in lung tissue derived from mice with bleomycin or adTGFβ1-induced pulmonary fibrosis. Mice were exposed to agents, and respective vehicle controls (con), and 3 weeks thereafter, lung tissues were homogenized for the assessment of pJNK via Western blot analysis. PBS or control-adenoviral vector exposed mice resulted in similar lack of p-JNK immunoreactivity (see Fig 1B below), and therefore only one representative control group (Con) is shown. (B) Immunolocalization of p-JNK (red) in lung tissue from mice 3 weeks after exposure to a representative vehicle control, bleomycin or adenovirus expressing recombinant active transforming growth factor beta-1 (adTGFβ1). Lower Panels; Co-localization of p-JNK with CCSP in control vehicle, bleomycin or adTGFβ1. Results were evaluated via confocal microscopy. Co-localization of p-JNK and CCSP is indicated by a yellow color. Scale bars: 50 μm. (C) p-JNK (blue) in lung tissue from patients with idiopathic pulmonary fibrosis (IPF) or non-IPF lung (Normal, n = 5 patients/group). Scale bars: 300 μm.

%I PLOS ONE