%0 Generic %A W. Powers, Thomas %A A. Neely, Benjamin %A Shao, Yuan %A Tang, Huiyuan %A Troyer, Dean A. %A Mehta, Anand S. %A Haab, Brian B. %A Drake, Richard R. %D 2014 %T MALDI Imaging Mass Spectrometry Profiling of N-Glycans in Formalin-Fixed Paraffin Embedded Clinical Tissue Blocks and Tissue Microarrays %U https://plos.figshare.com/articles/dataset/_MALDI_Imaging_Mass_Spectrometry_Profiling_of_N_Glycans_in_Formalin_Fixed_Paraffin_Embedded_Clinical_Tissue_Blocks_and_Tissue_Microarrays_/1159902 %R 10.1371/journal.pone.0106255 %2 https://ndownloader.figshare.com/files/1660017 %2 https://ndownloader.figshare.com/files/1660018 %2 https://ndownloader.figshare.com/files/1660019 %2 https://ndownloader.figshare.com/files/1660020 %2 https://ndownloader.figshare.com/files/1660021 %2 https://ndownloader.figshare.com/files/1660022 %2 https://ndownloader.figshare.com/files/1660023 %2 https://ndownloader.figshare.com/files/1660024 %K database comparisons %K tissue distributions %K throughput analysis %K tissue types %K tumor microarray format %K Mouse Kidney %K tissue microarray %K Clinical Tissue Blocks %K mouse kidney tissue sections %K hepatocellular carcinoma tissue microarray %K FFPE tissue block %K Differential tissue distribution %K MALDI Imaging Mass Spectrometry Profiling %K antigen retrieval %K Tissue Microarrays %K prostate cancers %K glycan %X

A recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in frozen tissues has been extended and improved for the direct analysis of glycans in clinically derived formalin-fixed paraffin-embedded (FFPE) tissues. Formalin-fixed tissues from normal mouse kidney, human pancreatic and prostate cancers, and a human hepatocellular carcinoma tissue microarray were processed by antigen retrieval followed by on-tissue digestion with peptide N-glycosidase F. The released N-glycans were detected by MALDI-IMS analysis, and the structural composition of a subset of glycans could be verified directly by on-tissue collision-induced fragmentation. Other structural assignments were confirmed by off-tissue permethylation analysis combined with multiple database comparisons. Imaging of mouse kidney tissue sections demonstrates specific tissue distributions of major cellular N-linked glycoforms in the cortex and medulla. Differential tissue distribution of N-linked glycoforms was also observed in the other tissue types. The efficacy of using MALDI-IMS glycan profiling to distinguish tumor from non-tumor tissues in a tumor microarray format is also demonstrated. This MALDI-IMS workflow has the potential to be applied to any FFPE tissue block or tissue microarray to enable higher throughput analysis of the global changes in N-glycosylation associated with cancers.

%I PLOS ONE