%0 Journal Article %A Park, Sung-Bin %A Hasegawa, Urara %A J. van der Vlies, André %A Sung, Moon-Hee %A Uyama, Hiroshi %D 2014 %T Preparation of poly(γ-glutamic acid)/hydroxyapatite monolith via biomineralization for bone tissue engineering %U https://tandf.figshare.com/articles/journal_contribution/Preparation_of_poly_947_glutamic_acid_hydroxyapatite_monolith_via_biomineralization_for_bone_tissue_engineering/1157697 %R 10.6084/m9.figshare.1157697.v2 %2 https://ndownloader.figshare.com/files/1657381 %K polystyrene culture plate %K PGA monolith %K bmp %K mtt %K macroporous cell scaffold %K Murine osteoblastic MC 3T cells %K Bone tissue engineering %K bimodal pore size distribution %X

A hybrid monolith of poly(γ-glutamic acid) and hydroxyapatite (PGA/HAp monolith) was prepared via biomineralization and used as a macroporous cell scaffold in bone tissue engineering. The PGA monolith having a bimodal pore size distribution was used as a substrate to induce biomineralization. The PGA/HAp monolith was obtained by immersing the PGA monolith in simulated body fluid. Pretreatment with CaCl2 enhanced the apatite-forming ability of the PGA monolith. Murine osteoblastic MC3T3-E1 cells efficiently attached and proliferated on the PGA/HAp monolith. MTT assay showed that both the PGA and PGA/HAp monolith did not have apparent cytotoxicity. Moreover, the PGA and PGA/HAp monoliths adsorbed bone morphogenetic protein-2 (BMP-2) by electrostatic interaction which was slowly released in the medium during cell culture. The PGA/HAp monolith enhanced BMP-2 induced alkaline phosphatase activity compared to the PGA monolith and a polystyrene culture plate. Thus, these PGA/HAp monoliths may have potential in bone tissue engineering.

%I Taylor & Francis