10.1371/journal.pone.0106243.g003 Amanda Haymond Amanda Haymond Chinchu Johny Chinchu Johny Tyrone Dowdy Tyrone Dowdy Brandon Schweibenz Brandon Schweibenz Karen Villarroel Karen Villarroel Richard Young Richard Young Clark J. Mantooth Clark J. Mantooth Trishal Patel Trishal Patel Jessica Bases Jessica Bases Geraldine San Jose Geraldine San Jose Emily R. Jackson Emily R. Jackson Cynthia S. Dowd Cynthia S. Dowd Robin D. Couch Robin D. Couch Purification of recombinant <i>Y. pestis</i> MEP synthase. Public Library of Science 2014 KMNADPH product allosteric affector FR 900098 KMDXP drug development DXP binding sites IC 50 values MEP pathway methylerythritol phosphate pestis MEP synthase pilot scale library nadph MEP synthase nm novel antibiotic development 2014-08-29 02:58:51 Figure https://plos.figshare.com/articles/figure/_Purification_of_recombinant_Y_pestis_MEP_synthase_/1155777 <p>A Coomassie stained SDS-PAGE shows two lanes of purified His-tagged MEP synthase alongside a molecular weight marker (MW). His-tagged MEP synthase has a predicted molecular weight of 46.7 kDa. The typical yield of purified protein averaged 30 mg per 1 L shake flask.</p>