10.1371/journal.pone.0106243.g003
Amanda Haymond
Amanda
Haymond
Chinchu Johny
Chinchu
Johny
Tyrone Dowdy
Tyrone
Dowdy
Brandon Schweibenz
Brandon
Schweibenz
Karen Villarroel
Karen
Villarroel
Richard Young
Richard
Young
Clark J. Mantooth
Clark
J. Mantooth
Trishal Patel
Trishal
Patel
Jessica Bases
Jessica
Bases
Geraldine San Jose
Geraldine
San Jose
Emily R. Jackson
Emily
R. Jackson
Cynthia S. Dowd
Cynthia S.
Dowd
Robin D. Couch
Robin D.
Couch
Purification of recombinant <i>Y. pestis</i> MEP synthase.
Public Library of Science
2014
KMNADPH
product allosteric affector
FR 900098
KMDXP
drug development
DXP binding sites
IC 50 values
MEP pathway
methylerythritol phosphate
pestis MEP synthase
pilot scale library
nadph
MEP synthase
nm
novel antibiotic development
2014-08-29 02:58:51
Figure
https://plos.figshare.com/articles/figure/_Purification_of_recombinant_Y_pestis_MEP_synthase_/1155777
<p>A Coomassie stained SDS-PAGE shows two lanes of purified His-tagged MEP synthase alongside a molecular weight marker (MW). His-tagged MEP synthase has a predicted molecular weight of 46.7 kDa. The typical yield of purified protein averaged 30 mg per 1 L shake flask.</p>