10.1371/journal.pone.0104272.g006
Arti Kumari
Arti
Kumari
Rani Gupta
Rani
Gupta
TEM analyses of recombinant P. pastoris under different physiological conditions showing differential peroxisome proliferation.
Public Library of Science
2014
carbon source
lipase
Lip 11
Pichia pastoris X 33
release methanol source
Slow Release Methanol Source
peroxisome proliferation studies
transmission electron microscopy
120 h
Lip C
methanol induction
AOX 1 promoter
methyl esters
2014-08-29 02:58:03
Figure
https://plos.figshare.com/articles/figure/_TEM_analyses_of_recombinant_P_pastoris_under_different_physiological_conditions_showing_differential_peroxisome_proliferation_/1155747
<p>a) Control in BMMY medium 48 h, no peroxisome is visible; b) methanol fed culture 48 h, larger peroxisomes were observed; c) oleic acid fed culture 48 h, smaller and numerous peroxisomes were present; d) mixed fed culture (methanol + oleic acid) 48 h, peroxisome of varying size were observed; e) methyl oleate fed cultures after 24 h, larger peroxisomes few in number; f) methyl oleate fed cultures after 72 h, peroxisomes of varying size were observed, g) methyl oleate fed cultures after 96 h, numerous peroxisomes of varying size were observed. The magnification is 1 µm for all images. N = nucleus, V = vacuole and P = peroxisome.</p>