10.1371/journal.pone.0104272.g006 Arti Kumari Arti Kumari Rani Gupta Rani Gupta TEM analyses of recombinant P. pastoris under different physiological conditions showing differential peroxisome proliferation. Public Library of Science 2014 carbon source lipase Lip 11 Pichia pastoris X 33 release methanol source Slow Release Methanol Source peroxisome proliferation studies transmission electron microscopy 120 h Lip C methanol induction AOX 1 promoter methyl esters 2014-08-29 02:58:03 Figure https://plos.figshare.com/articles/figure/_TEM_analyses_of_recombinant_P_pastoris_under_different_physiological_conditions_showing_differential_peroxisome_proliferation_/1155747 <p>a) Control in BMMY medium 48 h, no peroxisome is visible; b) methanol fed culture 48 h, larger peroxisomes were observed; c) oleic acid fed culture 48 h, smaller and numerous peroxisomes were present; d) mixed fed culture (methanol + oleic acid) 48 h, peroxisome of varying size were observed; e) methyl oleate fed cultures after 24 h, larger peroxisomes few in number; f) methyl oleate fed cultures after 72 h, peroxisomes of varying size were observed, g) methyl oleate fed cultures after 96 h, numerous peroxisomes of varying size were observed. The magnification is 1 µm for all images. N  =  nucleus, V =  vacuole and P =  peroxisome.</p>