10.1371/journal.pbio.1001434
Nitika Raychaudhuri
Nitika
Raychaudhuri
Raphaelle Dubruille
Raphaelle
Dubruille
Guillermo A. Orsi
Guillermo
A. Orsi
Homayoun C. Bagheri
Homayoun
C. Bagheri
Benjamin Loppin
Benjamin
Loppin
Christian F. Lehner
Christian
F. Lehner
Transgenerational Propagation and Quantitative Maintenance of Paternal Centromeres Depends on Cid/Cenp-A Presence in <em>Drosophila</em> Sperm
Public Library of Science
2012
transgenerational
propagation
quantitative
paternal
centromeres
depends
sperm
2012-12-27 01:28:15
Dataset
https://plos.figshare.com/articles/dataset/Transgenerational_Propagation_and_Quantitative_Maintenance_of_Paternal_Centromeres_Depends_on_Cid_Cenp_A_Presence_in_Drosophila_Sperm__/115295
<div><p>In <em>Drosophila melanogaster</em>, as in many animal and plant species, centromere identity is specified epigenetically. In proliferating cells, a centromere-specific histone H3 variant (CenH3), named Cid in Drosophila and Cenp-A in humans, is a crucial component of the epigenetic centromere mark. Hence, maintenance of the amount and chromosomal location of CenH3 during mitotic proliferation is important. Interestingly, CenH3 may have different roles during meiosis and the onset of embryogenesis. In gametes of <em>Caenorhabditis elegans</em>, and possibly in plants, centromere marking is independent of CenH3. Moreover, male gamete differentiation in animals often includes global nucleosome for protamine exchange that potentially could remove CenH3 nucleosomes. Here we demonstrate that the control of Cid loading during male meiosis is distinct from the regulation observed during the mitotic cycles of early embryogenesis. But Cid is present in mature sperm. After strong Cid depletion in sperm, paternal centromeres fail to integrate into the gonomeric spindle of the first mitosis, resulting in gynogenetic haploid embryos. Furthermore, after moderate depletion, paternal centromeres are unable to re-acquire normal Cid levels in the next generation. We conclude that Cid in sperm is an essential component of the epigenetic centromere mark on paternal chromosomes and it exerts quantitative control over centromeric Cid levels throughout development. Hence, the amount of Cid that is loaded during each cell cycle appears to be determined primarily by the preexisting centromeric Cid, with little flexibility for compensation of accidental losses.</p> </div>