10.1371/journal.pone.0103817.g001 Delyan P. Ivanov Delyan P. Ivanov Terry L. Parker Terry L. Parker David A. Walker David A. Walker Cameron Alexander Cameron Alexander Marianne B. Ashford Marianne B. Ashford Paul R. Gellert Paul R. Gellert Martin C. Garnett Martin C. Garnett Spheroid volume increase of NSC and UW228-3 cells as a function of initial seeding number. Public Library of Science 2014 spheroid volume determination culture method acid phosphatase enzyme activity anticancer drug screen spheroid health assessment Acid Phosphatase Viability Assays 3 D uw foetal brain tissue Multiplexing Spheroid Volume 2014-08-13 04:46:56 Figure https://plos.figshare.com/articles/figure/_Spheroid_volume_increase_of_NSC_and_UW228_3_cells_as_a_function_of_initial_seeding_number_/1138048 <p>Volume increase % = 100*(V<sub>day7</sub>−V<sub>day1</sub>)/V<sub>day1</sub>. NSCs grew more in a week than UW cells, reaching maximum growth increase of 600% for seeding 1000 cells per spheroid, whereas the maximum growth increase for tumour cells was around 170% for 2000–5000 cells/spheroid.</p>