10.1371/journal.pone.0104882
Jianfeng Wang
Jianfeng
Wang
Zhi Ruan
Zhi
Ruan
Ye Feng
Ye
Feng
Ying Fu
Ying
Fu
Yan Jiang
Yan
Jiang
Haiping Wang
Haiping
Wang
Yunsong Yu
Yunsong
Yu
Species Distribution of Clinical <i>Acinetobacter</i> Isolates Revealed by Different Identification Techniques
Public Library of Science
2014
genomic species 33 YU
Different Identification Techniques
Clinical Acinetobacter Isolates Revealed
Acinetobacter spp
phenotypic identification results
method
disk diffusion assay
novel Acinetobacter species
ms
vitek
baumannii
rpoB gene sequencing
antimicrobial susceptibility test
16 S rRNA
2014-08-13 04:46:11
Dataset
https://plos.figshare.com/articles/dataset/Species_Distribution_of_Clinical_Acinetobacter_Isolates_Revealed_by_Different_Identification_Techniques/1138041
<div><p>A total of 2582 non-duplicate clinical <i>Acinetobacter</i> spp. isolates were collected to evaluate the performance of four identification methods because it is important to identify <i>Acinetobacter</i> spp. accurately and survey the species distribution to determine the appropriate antimicrobial treatment. Phenotyping (VITEK 2 and VITEK MS) and genotyping (16S rRNA and <i>rpoB</i> gene sequencing) methods were applied for species identification, and antimicrobial susceptibility test of imipenem and meropenem was performed with a disk diffusion assay. Generally, the phenotypic identification results were quite different from the genotyping results, and their discrimination ability was unsatisfactory, whereas 16S rRNA and <i>rpoB</i> gene sequencing showed consistent typing results, with different resolution. Additionally, <i>A. pittii</i>, <i>A. calcoaceticus</i> and <i>A. nosocomialis</i>, which were phylogenetically close to <i>A. baumannii</i>, accounted for 85.5% of the non-<i>A. baumannii</i> isolates. One group, which could not be clustered with any reference strains, consisted of 11 isolates and constituted a novel <i>Acinetobacter</i> species that was entitled <i>genomic species 33YU</i>. None of the non-<i>A. baumannii</i> isolates harbored a <i>bla</i><sub>OXA-51</sub>-like gene, and this gene was disrupted by IS<i>Aba19</i> in only one isolate; it continues to be appropriate as a genetic marker for <i>A. baumannii</i> identification. The resistance rate of <i>non-A. baumannii</i> isolates to imipenem and/or meropenem was only 2.6%, which was significantly lower than that of <i>A. baumannii</i>. Overall, <i>rpoB</i> gene sequencing was the most accurate identification method for <i>Acinetobacter</i> species. Except for <i>A. baumannii</i>, the most frequently isolated species from the nosocomial setting were <i>A. pittii</i>, <i>A. calcoaceticus</i> and <i>A. nosocomialis</i>.</p></div>