MOESM1 of Adult human cardiac stem cell supplementation effectively increases contractile function and maturation in human engineered cardiac tissues
Jack Murphy
Joshua Mayourian
Francesca Stillitano
Sadek Munawar
Kathleen Broughton
Esperanza Agullo-Pascual
Mark Sussman
Roger Hajjar
Kevin Costa
Irene Turnbull
10.6084/m9.figshare.11321666.v1
https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_Adult_human_cardiac_stem_cell_supplementation_effectively_increases_contractile_function_and_maturation_in_human_engineered_cardiac_tissues/11321666
Additional file 1: Figure S1. Representative image of hCSC-supplemented hECT fabricated with hCSCs labeled with Qtracker-655 and co-stained with cardiac troponin T. A) Confocal max projection image showing cardiac marker Troponin T (green), hCSCs labeled with Qtracker-655 (magenta), and nuclei stained with DAPI (blue); arrowheads point to the presence of Qtracker, scale bar = 50 μm. B, C) Zoom in of inset from panel A. B) Is a Max projection of with orthogonal views, scale bar = 5 μm. C) Single slice of the same inset, with orthogonal views. The orthogonal views of the max projection (in B) and the single slice (in C) show that there is no colocalization of Qtracker and Troponin T; where Qtracker positive cells appear adjacent but distinct from troponin T positive cells.
2019-12-05 05:30:37
Human cardiac stem cells
Human engineered cardiac tissue
Contractility
Myocardial function
Cardiomyocyte maturation