10.1371/journal.pone.0104045.g006
Stefania Briganti
Stefania
Briganti
Enrica Flori
Enrica
Flori
Barbara Bellei
Barbara
Bellei
Mauro Picardo
Mauro
Picardo
Possible interference of PPARĪ³ against PUVA induced modulation of the cellular stress response system.
Public Library of Science
2014
Biochemistry
enzymology
Enzyme chemistry
Enzyme regulation
enzymes
hydrolases
Collagenases
lipids
Lipid structure
sterols
cytochemistry
cell biology
Cellular structures and organelles
Cell membranes
Membrane characteristics
membrane composition
dermatology
Photodermatology and skin aging
interference
puva
induced
modulation
cellular
2014-08-07 03:06:39
Figure
https://plos.figshare.com/articles/figure/_Possible_interference_of_PPAR_947_against_PUVA_induced_modulation_of_the_cellular_stress_response_system_/1131302
<p>(A) RT-PCR was performed to measure the expression of NRF2 mRNA 6 and 24 h after PUVA exposure, w/o Octa post-incubation. The level of NRF2 mRNA was normalized to the expression of GAPDH and is expressed relative to untreated control cells (**p<0.001 respect to Ctr; <sup>##</sup>p<0.001 compared with PUVA-treated fibroblasts). (B) RT-PCR was performed to measure the expression of HO-1 mRNA 6 and 24 h after PUVA exposure, w/o Octa post-incubation. The level of HO-1 mRNA was normalized to the expression of GAPDH and is expressed relative to untreated control cells (**p<0.001 respect to Ctr; <sup>##</sup>p<0.001 compared with PUVA-treated fibroblasts). (C) GSH concentrations were determined by HPLC-MS) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104045#s2" target="_blank"><b>Materials and Methods</b></a> (*p<0.05 respect to control fibroblasts). (D) RT-PCR was performed to measure the expression of FoxO1 mRNA 6 and 24 h after PUVA exposure, w/o Octa post-incubation. The level of FoxO1 mRNA was normalized to the expression of GAPDH and is expressed relative to untreated control cells. *p<0.05 respect to control fibroblasts; <sup>#</sup>p<0.05 compared with PUVA-treated fibroblasts.</p>