10.1371/journal.pone.0104045.g006 Stefania Briganti Stefania Briganti Enrica Flori Enrica Flori Barbara Bellei Barbara Bellei Mauro Picardo Mauro Picardo Possible interference of PPARĪ³ against PUVA induced modulation of the cellular stress response system. Public Library of Science 2014 Biochemistry enzymology Enzyme chemistry Enzyme regulation enzymes hydrolases Collagenases lipids Lipid structure sterols cytochemistry cell biology Cellular structures and organelles Cell membranes Membrane characteristics membrane composition dermatology Photodermatology and skin aging interference puva induced modulation cellular 2014-08-07 03:06:39 Figure https://plos.figshare.com/articles/figure/_Possible_interference_of_PPAR_947_against_PUVA_induced_modulation_of_the_cellular_stress_response_system_/1131302 <p>(A) RT-PCR was performed to measure the expression of NRF2 mRNA 6 and 24 h after PUVA exposure, w/o Octa post-incubation. The level of NRF2 mRNA was normalized to the expression of GAPDH and is expressed relative to untreated control cells (**p<0.001 respect to Ctr; <sup>##</sup>p<0.001 compared with PUVA-treated fibroblasts). (B) RT-PCR was performed to measure the expression of HO-1 mRNA 6 and 24 h after PUVA exposure, w/o Octa post-incubation. The level of HO-1 mRNA was normalized to the expression of GAPDH and is expressed relative to untreated control cells (**p<0.001 respect to Ctr; <sup>##</sup>p<0.001 compared with PUVA-treated fibroblasts). (C) GSH concentrations were determined by HPLC-MS) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104045#s2" target="_blank"><b>Materials and Methods</b></a> (*p<0.05 respect to control fibroblasts). (D) RT-PCR was performed to measure the expression of FoxO1 mRNA 6 and 24 h after PUVA exposure, w/o Octa post-incubation. The level of FoxO1 mRNA was normalized to the expression of GAPDH and is expressed relative to untreated control cells. *p<0.05 respect to control fibroblasts; <sup>#</sup>p<0.05 compared with PUVA-treated fibroblasts.</p>