%0 Figure %A You, Xiaoxing %A Liu, Liangzhuan %A Zeng, Yanhua %A Li, Ranhui %A He, Jun %A Ma, Xiaohua %A Jiang, Chuanhao %A Zhu, Cuiming %A Chen, Liesong %A Yu, Minjun %A Ou, Guangli %A Wu, Yimou %D 2014 %T MALP-2 induces PI3K activation is regulated by the formation of a Btk, Mal, c-Src and p85α complex. %U https://plos.figshare.com/articles/figure/_MALP_2_induces_PI3K_activation_is_regulated_by_the_formation_of_a_Btk_Mal_c_Src_and_p85_945_complex_/1123066 %R 10.1371/journal.pone.0103433.g007 %2 https://ndownloader.figshare.com/files/1616395 %K cell biology %K Cellular types %K Animal cells %K Immune cells %K immunology %K Immunity to infections %K PI3K %K activation %K regulated %K c-src %X

A. Cells were pretreated with or without LFM-A13 (100 µM), or PP1 (50 µM) for 1 h, and then incubated in the absence or presence of 5 ng/ml MALP-2 for 15 min. Cell fractions were prepared and subjected to Western blotting analysis with an anti-p-Akt antibody. Data shown represents three experiments (means ± SEM). *, P<0.05 and **, P<0.01 for significant difference between compared groups. B, C. Cells were transfected with siRNA of Mal or MyD88, and then treated with MALP-2 for 15, 60 or 120 min. Phosphorylated Akt was detected by Western blotting. *, P<0.05 for significant difference between compared groups. D. Cells were treated with MALP-2 for the indicated times, the cell lysates were subjected to immunoprecipitation using an anti-c-Src or anti-p85α antibody, and followed by immunoblotting with anti-Btk, anti-Mal, anti-MyD88, anti-c-Src or anti p85α antibody. All figures are representative of three independent experiments.

%I PLOS ONE