TY - DATA T1 - Two different approaches for the calculation of the endocytosis time constant. PY - 2014/07/31 AU - Mareike Röther AU - Jan M. Brauner AU - Katrin Ebert AU - Oliver Welzel AU - Jasmin Jung AU - Anna Bauereiss AU - Johannes Kornhuber AU - Teja W. Groemer UR - https://plos.figshare.com/articles/figure/_Two_different_approaches_for_the_calculation_of_the_endocytosis_time_constant_/1123064 DO - 10.1371/journal.pone.0102723.g005 L4 - https://ndownloader.figshare.com/files/1616393 KW - anatomy KW - nervous system KW - Biochemistry KW - Neurochemistry KW - Neurochemicals KW - neurotransmitters KW - cell biology KW - Cell physiology KW - Membrane trafficking KW - Molecular cell biology KW - neuroscience KW - organisms KW - animals KW - vertebrates KW - mammals KW - rodents KW - rats KW - Model organisms KW - Animal models KW - Specimen preparation and treatment KW - Mechanical treatment of specimens KW - Specimen disruption KW - electroporation KW - approaches KW - endocytosis N2 - (A) Deconvolution of the reacidification time course from the alkaline vesicle population dynamics and estimated endocytosis time course. For analysis, experimental data from the action potential number series was used (stimulation paradigms: 200 pulses at 20 Hz). Stimulus begin was at 40 seconds. (B) Calculation of the time course of the surface pool, which is determined by the alkaline vesicle population (fluorescence time course at pH 5.5) subtracted from the total pHluorin (fluorescence time course at pH 7.5). The endocytosis time constants were calculated by monoexponential curve fitting. Stimulus begin was at 40 seconds. ER -