%0 Figure %A Cadavez, Lisa %A Montane, Joel %A Alcarraz-Vizán, Gema %A Visa, Montse %A Vidal-Fàbrega, Laia %A Servitja, Joan-Marc %A Novials, Anna %D 2014 %T Thapsigargin induces ER stress and apoptosis in hIAPP-INS1E cells. %U https://plos.figshare.com/articles/figure/_Thapsigargin_induces_ER_stress_and_apoptosis_in_hIAPP_INS1E_cells_/1099561 %R 10.1371/journal.pone.0101797.g001 %2 https://ndownloader.figshare.com/files/1587846 %K Biochemistry %K proteins %K Chaperone proteins %K cell biology %K Cell processes %K Cellular stress responses %K Endoplasmic reticulum stress response %K Metabolic disorders %K Diabetes mellitus %K type 2 diabetes %K Precursors of type 2 diabetes %K Pathology and laboratory medicine %K Signs and symptoms %K amyloidosis %K er %K apoptosis %K hiapp-ins1e %X

A) INS1E control cells were cultured at 11 mM glucose and treated with 1 µM of thapsigargin for 8 and 24 hours. mRNA levels of ER stress markers ATF3, sXBP1 and CHOP were quantified by Real-Time PCR. *p<0.05, **p<0.01 and ***p<0.001 versus control cells at time 0. Results are expressed s mean ± S.E.M from five independent experiments. B) INS1E and hIAPP cells were exposed to 1 µM thapsigargin for 24 hours. Expression levels of CHOP, cleaved caspase 3, insulin, IAPP and β-actin were determined by Western blot. C) INS1E and hIAPP-INS1E control cells were exposed to 0.25, 0.5 and 1 µM of thapsigargin (Thp) for 8 and 24 hours. Protein levels of CHOP, cleaved caspase 3, IAPP and β-actin were determined by Western blot (n = 3). Representative Western blotting images are shown from 2 to 3 independent experiments.

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