Thapsigargin induces ER stress and apoptosis in hIAPP-INS1E cells. Lisa Cadavez Joel Montane Gema Alcarraz-Vizán Montse Visa Laia Vidal-Fàbrega Joan-Marc Servitja Anna Novials 10.1371/journal.pone.0101797.g001 https://plos.figshare.com/articles/figure/_Thapsigargin_induces_ER_stress_and_apoptosis_in_hIAPP_INS1E_cells_/1099561 <p>A) INS1E control cells were cultured at 11 mM glucose and treated with 1 µM of thapsigargin for 8 and 24 hours. mRNA levels of ER stress markers ATF3, sXBP1 and CHOP were quantified by Real-Time PCR. *p<0.05, **p<0.01 and ***p<0.001 versus control cells at time 0. Results are expressed s mean ± S.E.M from five independent experiments. B) INS1E and hIAPP cells were exposed to 1 µM thapsigargin for 24 hours. Expression levels of CHOP, cleaved caspase 3, insulin, IAPP and β-actin were determined by Western blot. C) INS1E and hIAPP-INS1E control cells were exposed to 0.25, 0.5 and 1 µM of thapsigargin (Thp) for 8 and 24 hours. Protein levels of CHOP, cleaved caspase 3, IAPP and β-actin were determined by Western blot (n = 3). Representative Western blotting images are shown from 2 to 3 independent experiments.</p> 2014-07-10 03:14:57 Biochemistry proteins Chaperone proteins cell biology Cell processes Cellular stress responses Endoplasmic reticulum stress response Metabolic disorders Diabetes mellitus type 2 diabetes Precursors of type 2 diabetes Pathology and laboratory medicine Signs and symptoms amyloidosis er apoptosis hiapp-ins1e