Statin treatment and <i>P. aeruginosa</i> infection modulate the host immune response. Emma Hennessy Julie O'Callaghan Marlies J. Mooij Claire Legendre Olga Camacho-Vanegas Sandra C. Camacho Claire Adams John A. Martignetti Fergal O'Gara 10.1371/journal.pone.0102200.g001 https://plos.figshare.com/articles/figure/_Statin_treatment_and_P_aeruginosa_infection_modulate_the_host_immune_response_/1099267 <p>A549 cells were treated with 10 µM simvastatin or an equivalent amount of DMSO (VEHICLE) for 24 hours. Untreated and statin-treated cells were subsequently infected with <i>P. aeruginosa</i> PAO1 for 3 hours at MOI 50∶1. The expression of (<b>A</b>) KLF2 (<b>B</b>) KLF6 (<b>C</b>) IL-8 (<b>D</b>) CCL20 and (<b>E</b>) TLR5 was analysed in uninfected cells, and in the presence of vehicle, simvastatin (SIM), <i>P. aeruginosa</i> (PAO1) and simvastatin and PAO1 combined (SIM+PAO1). *<i>P</i>, ≤0.05, **<i>P</i>, ≤0.01, ***<i>P</i>, ≤0.001.</p> 2014-07-10 02:59:17 cell biology Molecular cell biology genetics gene expression immunology Immune response inflammation Immune system Innate immune system immunity Immune activation microbiology bacteriology Gram negative bacteria Medical microbiology Microbial pathogens Bacterial pathogens Infectious diseases Bacterial diseases Pseudomonas infections Pathology and laboratory medicine pathogenesis Host-pathogen interactions modulate