%0 Figure %A Logez, Christel %A Berger, Sylvie %A Legros, Céline %A Banères, Jean-Louis %A Cohen, William %A Delagrange, Philippe %A Nosjean, Olivier %A Boutin, Jean A. %A Ferry, Gilles %A Simonin, Frédéric %A Wagner, Renaud %D 2014 %T MT1 expression yields obtained with ten combinations of cDNA constructs and host systems. %U https://plos.figshare.com/articles/figure/_MT1_expression_yields_obtained_with_ten_combinations_of_cDNA_constructs_and_host_systems_/1082100 %R 10.1371/journal.pone.0100616.g001 %2 https://ndownloader.figshare.com/files/1563338 %K Biochemistry %K proteins %K protein structure %K Recombinant proteins %K cytochemistry %K Protein chemistry %K cell biology %K Cellular structures and organelles %K Cell membranes %K Membrane proteins %K yields %K combinations %K cdna %K constructs %X

Expression levels of the human MT1 receptor were assessed using a [3H]-melatonin ligand binding assay. N: number of independent experiments. Schematic representations of the evaluated expression vectors use the following abbreviations: MT1, human MT1 receptor; His, 10-histidine tag; attB1 and attB2, recombination sites of the Gateway system; Tev, tobacco etch virus protease cleavage site; Trx protein, thioredoxin protein; α-F, sequence signal of the Saccharomyces cerevisiae α-Factor; Flag, flag-epitope tag; Biotin, biotinylation domain from Propionibacterium shermanii; Gαi1, αi1 subunit of G protein; ss, signal sequence from influenza hemagglutinin gene; YFP, yellow fluorescent protein; and 2-Strep, double Strep tag.

%I PLOS ONE