10.1371/journal.pbio.1001893.g001 Andreas Schoofs Andreas Schoofs Sebastian Hückesfeld Sebastian Hückesfeld Philipp Schlegel Philipp Schlegel Anton Miroschnikow Anton Miroschnikow Marc Peters Marc Peters Malou Zeymer Malou Zeymer Roland Spieß Roland Spieß Ann-Shyn Chiang Ann-Shyn Chiang Michael J. Pankratz Michael J. Pankratz Identification of neuronal networks modulating motor patterns using Gal4-directed thermo-sensitive UAS-dTrpA1 expression. Public Library of Science 2014 neuroscience neuronal networks modulating patterns gal4-directed thermo-sensitive uas-dtrpa1 2014-06-24 03:22:56 Figure https://plos.figshare.com/articles/figure/_Identification_of_neuronal_networks_modulating_motor_patterns_using_Gal4_directed_thermo_sensitive_UAS_dTrpA1_expression_/1081454 <p>(A) Experimental setup for AN, MN, and PaN recordings at the deafferented CNS; dTrpA1 was activated by a Peltier-driven heating device. (B) Single extracellular recordings of AN, MN, and PaN revealed differential alteration of feeding-related motor patterns by dTrpA1 activation. Red blocks on top of the control recordings denote motor output. For the experimental recordings, an up arrow (↑) indicates significant acceleration of motor pattern, down arrow (↓) indicates significant deceleration of motor pattern and a dash (–) indicates no significant difference in the motor pattern (exception: Glu (Ok371-Gal4 showed no rhythmic motor pattern by dTrpA1 activation [no]). (C) Statistical data from AN, MN, and PaN motor patterns quantified as relative change in cycle frequency (mean ± standard error). Significance was tested by Mann-Whitney Rank Sum Test (***p≤0.001). 5-HT, serotonin; ACh, acetylcholine; DA, dopamine; Glu, glutamate; Hug, hugin neuropeptide; MHD, mouth hook depressor; MHE, mouth hook elevator; Pro<sub>do</sub>A, dorsal protractor A.</p>