10.1371/journal.pone.0070712.g002 Jaspreet Singh Jaspreet Singh Mushfiquddin Khan Mushfiquddin Khan Aurora Pujol Aurora Pujol Mauhamad Baarine Mauhamad Baarine Inderjit Singh Inderjit Singh SAHA treatment upregulates Abcd2 and Abcd3 levels in Abcd1-deficient U87 astrocytes and B12 oligodendrocytes. Public Library of Science 2013 Biochemistry lipids Fatty acids Lipid metabolism neuroscience Molecular neuroscience Signaling pathways neurology Demyelinating disorders Developmental and pediatric neurology Neurodegenerative diseases upregulates abcd2 abcd3 abcd1-deficient u87 astrocytes b12 2013-07-26 09:59:19 Figure https://plos.figshare.com/articles/figure/_SAHA_treatment_upregulates_Abcd2_and_Abcd3_levels_in_Abcd1_deficient_U87_astrocytes_and_B12_oligodendrocytes_/1072926 <p>There was no change in Abcd1 protein levels (<b>A-i and E-i</b>) and mRNA expression (<b>A-ii and E-ii</b>) in Abcd1-deficient U87 astrocytes and B12 oligodendrocytes treated with SAHA. Abcd2 (<b>B and F</b>) and Abcd3 (<b>C and G</b>) protein levels (<b>i</b>) and mRNA expression (<b>ii</b>) in control, NT and Abcd1-deficient U87 astrocytes and B12 oligodendrocytes respectively (n = 3) treated with SAHA dose-dependently. Protein levels of the peroxisomal transporters Abcd1 (<b>A-i and E-i</b>), Abcd2 (<b>B-i and F-i</b>) and Abcd3 (<b>C-i and G-i</b>) were analyzed by Western analysis of peroxisomal membrane fractions obtained by carbonate treatment (membrane preparation containing integral membrane proteins), as indicated in Methods section. Na<sup>+</sup>/K<sup>+</sup>-ATPase (plasma membrane protein) was used as indicator of protein loading for plasma membrane fractions. Abcd1 (<b>A-ii and E-ii</b>), Abcd2 (<b>B-ii and F-ii</b>) and Abcd3 (<b>C-ii and G-ii</b>) mRNA levels were determined by qRT–PCR and normalized to GAPDH. Data are represented as mean±SD. <sup>@</sup>P<0.001 Abcd1-Lenti compared to control or NT cells; **P<0.001 SAHA (5.0 µM) treatment compared to Abcd1-Lenti; *P<0.005 SAHA treatment compared to Abcd1-Lenti; <sup>@@</sup>P<0.001 SAHA treatment compared to Abcd1-Lenti; NS, non-significant; SA, SAHA.</p>