10.1371/journal.pone.0070712.g002
Jaspreet Singh
Jaspreet
Singh
Mushfiquddin Khan
Mushfiquddin
Khan
Aurora Pujol
Aurora
Pujol
Mauhamad Baarine
Mauhamad
Baarine
Inderjit Singh
Inderjit
Singh
SAHA treatment upregulates Abcd2 and Abcd3 levels in Abcd1-deficient U87 astrocytes and B12 oligodendrocytes.
Public Library of Science
2013
Biochemistry
lipids
Fatty acids
Lipid metabolism
neuroscience
Molecular neuroscience
Signaling pathways
neurology
Demyelinating disorders
Developmental and pediatric neurology
Neurodegenerative diseases
upregulates
abcd2
abcd3
abcd1-deficient
u87
astrocytes
b12
2013-07-26 09:59:19
Figure
https://plos.figshare.com/articles/figure/_SAHA_treatment_upregulates_Abcd2_and_Abcd3_levels_in_Abcd1_deficient_U87_astrocytes_and_B12_oligodendrocytes_/1072926
<p>There was no change in Abcd1 protein levels (<b>A-i and E-i</b>) and mRNA expression (<b>A-ii and E-ii</b>) in Abcd1-deficient U87 astrocytes and B12 oligodendrocytes treated with SAHA. Abcd2 (<b>B and F</b>) and Abcd3 (<b>C and G</b>) protein levels (<b>i</b>) and mRNA expression (<b>ii</b>) in control, NT and Abcd1-deficient U87 astrocytes and B12 oligodendrocytes respectively (n = 3) treated with SAHA dose-dependently. Protein levels of the peroxisomal transporters Abcd1 (<b>A-i and E-i</b>), Abcd2 (<b>B-i and F-i</b>) and Abcd3 (<b>C-i and G-i</b>) were analyzed by Western analysis of peroxisomal membrane fractions obtained by carbonate treatment (membrane preparation containing integral membrane proteins), as indicated in Methods section. Na<sup>+</sup>/K<sup>+</sup>-ATPase (plasma membrane protein) was used as indicator of protein loading for plasma membrane fractions. Abcd1 (<b>A-ii and E-ii</b>), Abcd2 (<b>B-ii and F-ii</b>) and Abcd3 (<b>C-ii and G-ii</b>) mRNA levels were determined by qRT–PCR and normalized to GAPDH. Data are represented as mean±SD. <sup>@</sup>P<0.001 Abcd1-Lenti compared to control or NT cells; **P<0.001 SAHA (5.0 µM) treatment compared to Abcd1-Lenti; *P<0.005 SAHA treatment compared to Abcd1-Lenti; <sup>@@</sup>P<0.001 SAHA treatment compared to Abcd1-Lenti; NS, non-significant; SA, SAHA.</p>