%0 Figure %A Zhang, Ming %A Xu, Ming-xi %A Zhou, Zhe %A Zhang, Ke %A Zhou, Juan %A Zhao, Yang %A Wang, Zhong %A Lu, Mu-jun %D 2014 %T Urothelium-like phenotype differentiation of ASCs. %U https://plos.figshare.com/articles/figure/_Urothelium_like_phenotype_differentiation_of_ASCs_/1072911 %R 10.1371/journal.pone.0095583.g002 %2 https://ndownloader.figshare.com/files/1552879 %K biotechnology %K Bioengineering %K Tissue engineering %K cell biology %K Cell physiology %K Molecular cell biology %K developmental biology %K Molecular development %K cytokines %K Cell differentiation %K genetics %K gene expression %K urology %K phenotype %K differentiation %X

Immunofluorescent staining detection of UP-IA and UP-II in differentiated ASCs. The differentiation efficiency of induction by conditioned medium and transwell system for 7 and 21 days. The quantification of mRNA for the urothelium-specific marker UP-IA in ASCs after induction by conditioned media for 7 and 21 days. A, G: UP-IA and UP-II expression in urothelium. B, H: ASCs did not express UP-IA or UP-II. C, I, E, K: ASCs expressed UP-IA and UP-II in a small amount after induction by CM or transwell system for 7 days. D, J, F, L: UP-IA and UP-II expression in ASCs after induction for 21 days by conditioned medium or transwell system. M: The difference in the ASC differentiation efficiency between the two different induction methods at the 7th and 21st day. N: The quantification of UP-IA mRNA in human ASCs after induction by conditioned media for 7 and 21 days. CM = conditioned medium, TW = transwell indirect co-culture, Scale bar = 20 µm.

%I PLOS ONE