3′ UTR <i>SLC11A1</i> gene polymorphism analysis in resistant and susceptible macrophages.
Hugo Esquivel-Solís
Antonio J. Vallecillo
Alejandro Benítez-Guzmán
L. Garry Adams
Yolanda López-Vidal
José A. Gutiérrez-Pabello
10.1371/journal.pone.0063464.g002
https://plos.figshare.com/articles/figure/_3_UTR_SLC11A1_gene_polymorphism_analysis_in_resistant_and_susceptible_macrophages_/1068411
<p>Representative results from resistant (R) and susceptible (S) cattle genomic DNA single-stranded conformational analysis (SSCA) indicating the homozygous GT13 (175 bp) and heterozygous GT13/GT14 (177 bp) alleles. DNA from cattle genetically characterized as susceptible (SLC11A1s) or resistant (SLC11A1r) to standardized <i>Brucella abortus</i> in vivo challenge were used as controls (Cx) (A). DNA sequencing of PCR amplicons of <i>SLC11A1</i> 3′UTR confirmed the SSCA results evidencing the only one heterozygous genotype among all phenotyped susceptible and resistant cattle to BCG (B).</p>
2013-05-15 07:34:50
Biochemistry
Neurochemistry
Neurochemicals
Nitric oxide
Evolutionary biology
population genetics
Genetic polymorphism
genetics
immunology
Immune cells
monocytes
immunity
Innate immunity
neuroscience
Veterinary diseases
Zoonotic diseases
Bovine tuberculosis
Veterinary medicine
Veterinary immunology
utr
polymorphism
resistant
susceptible