3′ UTR <i>SLC11A1</i> gene polymorphism analysis in resistant and susceptible macrophages. Hugo Esquivel-Solís Antonio J. Vallecillo Alejandro Benítez-Guzmán L. Garry Adams Yolanda López-Vidal José A. Gutiérrez-Pabello 10.1371/journal.pone.0063464.g002 https://plos.figshare.com/articles/figure/_3_UTR_SLC11A1_gene_polymorphism_analysis_in_resistant_and_susceptible_macrophages_/1068411 <p>Representative results from resistant (R) and susceptible (S) cattle genomic DNA single-stranded conformational analysis (SSCA) indicating the homozygous GT13 (175 bp) and heterozygous GT13/GT14 (177 bp) alleles. DNA from cattle genetically characterized as susceptible (SLC11A1s) or resistant (SLC11A1r) to standardized <i>Brucella abortus</i> in vivo challenge were used as controls (Cx) (A). DNA sequencing of PCR amplicons of <i>SLC11A1</i> 3′UTR confirmed the SSCA results evidencing the only one heterozygous genotype among all phenotyped susceptible and resistant cattle to BCG (B).</p> 2013-05-15 07:34:50 Biochemistry Neurochemistry Neurochemicals Nitric oxide Evolutionary biology population genetics Genetic polymorphism genetics immunology Immune cells monocytes immunity Innate immunity neuroscience Veterinary diseases Zoonotic diseases Bovine tuberculosis Veterinary medicine Veterinary immunology utr polymorphism resistant susceptible