10.1371/journal.pntd.0002955
Ponpan Matangkasombut
Ponpan
Matangkasombut
Wilawan Chan-in
Wilawan
Chan-in
Anunya Opasawaschai
Anunya
Opasawaschai
Pisut Pongchaikul
Pisut
Pongchaikul
Nattaya Tangthawornchaikul
Nattaya
Tangthawornchaikul
Sirijitt Vasanawathana
Sirijitt
Vasanawathana
Wannee Limpitikul
Wannee
Limpitikul
Prida Malasit
Prida
Malasit
Thaneeya Duangchinda
Thaneeya
Duangchinda
Gavin Screaton
Gavin
Screaton
Juthathip Mongkolsapaya
Juthathip
Mongkolsapaya
Invariant NKT Cell Response to Dengue Virus Infection in Human
Public Library of Science
2014
immunology
Immune system
Innate immune system
Clinical immunology
Immune response
immunity
Immunity to infections
microbiology
Virology
Emerging viral diseases
Medical microbiology
Infectious diseases
Viral diseases
Dengue fever
Emerging infectious diseases
Tropical diseases
neglected tropical diseases
nkt
dengue
2014-06-19 15:03:02
Dataset
https://plos.figshare.com/articles/dataset/_Invariant_NKT_Cell_Response_to_Dengue_Virus_Infection_in_Human_/1063956
<div><p>Background</p><p>Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis, the roles of earlier innate events remain largely uninvestigated. Invariant natural killer T (iNKT) cells represent innate-like T cells that could dictate subsequent adaptive response but their role in human dengue virus infection is not known. We hypothesized that iNKT cells play a role in human dengue infection.</p><p>Methods</p><p>Blood samples from a well-characterized cohort of children with DF, DHF, in comparison to non-dengue febrile illness (OFI) and healthy controls at various time points were studied. iNKT cells activation were analyzed by the expression of CD69 by flow cytometry. Their cytokine production was then analyzed after α-GalCer stimulation. Further, the CD1d expression on monocytes, and CD69 expression on conventional T cells were measured.</p><p>Results</p><p>iNKT cells were activated during acute dengue infection. The level of iNKT cell activation associates with the disease severity. Furthermore, these iNKT cells had altered functional response to subsequent <i>ex vivo</i> stimulation with α-GalCer. Moreover, during acute dengue infection, monocytic CD1d expression was also upregulated and conventional T cells also became activated.</p><p>Conclusion</p><p>iNKT cells might play an early and critical role in the pathogenesis of severe dengue viral infection in human. Targeting iNKT cells and CD1d serve as a potential therapeutic strategy for severe dengue infection in the future.</p></div>