%0 Figure %A Okumura, Toshiya %A Harada, Kae %A Oue, Kana %A Zhang, Jun %A Asano, Satoshi %A Hayashiuchi, Masaki %A Mizokami, Akiko %A Tanaka, Hiroto %A Irifune, Masahiro %A Kamata, Nobuyuki %A Hirata, Masato %A Kanematsu, Takashi %D 2014 %T PP2A and PP1 are translocated from the cytosol to lipid droplets in adipocytes in response to adrenaline stimulation. %U https://plos.figshare.com/articles/figure/_PP2A_and_PP1_are_translocated_from_the_cytosol_to_lipid_droplets_in_adipocytes_in_response_to_adrenaline_stimulation_/1063235 %R 10.1371/journal.pone.0100559.g004 %2 https://ndownloader.figshare.com/files/1541912 %K Biochemistry %K lipids %K fats %K Lipid metabolism %K metabolism %K pp1 %K translocated %K cytosol %K lipid %K droplets %K adipocytes %K adrenaline %X

(A–C) Translocation of PP2A (A, B) and PP1 (A, C) to the lipid droplet fraction in response to stimulation with 1 µM adrenaline (Adrn). The bar graph shows the amount of PP2A (B) and PP1 (C) in fat (black) and sup (white) fractions, respectively. A typical image from four independent experiments is shown (A). (D, E) Phosphatase activity in floating fat-cake fraction. Phosphatase activities of PP2A (D, n = 4) and PP1 (E, n = 5) were measured using floating fat-cake fractions from WT and PRIP-DKO explants treated with or without 1 µM adrenaline. The data represent mean ±SEM; *P<0.05, **P<0.01, ***P<0.001 and n.s. (not significant) versus the corresponding values for paired bars connected by solid and dotted lines (B–E).

%I PLOS ONE