TY - DATA T1 - FH535 reduces cyclin D1 and Survivin protein levels in Huh7 cells. PY - 2014/06/18 AU - Roberto Gedaly AU - Roberto Galuppo AU - Michael F. Daily AU - Malay Shah AU - Erin Maynard AU - Changguo Chen AU - Xiping Zhang AU - Karyn A. Esser AU - Donald A. Cohen AU - B. Mark Evers AU - Jieyun Jiang AU - Brett T. Spear UR - https://plos.figshare.com/articles/figure/_FH535_reduces_cyclin_D1_and_Survivin_protein_levels_in_Huh7_cells_/1061196 DO - 10.1371/journal.pone.0099272.g006 L4 - https://ndownloader.figshare.com/files/1539273 KW - cell biology KW - Signal transduction KW - cell signaling KW - Signaling cascades KW - Wnt signaling cascade KW - Beta-catenin signaling KW - Molecular cell biology KW - genetics KW - gene expression KW - Gastroenterology and hepatology KW - Liver diseases KW - Hepatocellular carcinoma KW - oncology KW - Cancers and neoplasms KW - Gastrointestinal tumors KW - cyclin KW - d1 KW - survivin KW - huh7 N2 - Huh7 cells were treated with DMSO alone or increasing amounts of FH535 for 38-PAGE, and transferred for Western analysis with antibodies against Cyclin D1, Survivin, and β-actin. The top of shows the western blot image; the bottom graph shows densitometric analysis of the western data. This densitometric analysis indicated that FH535 at 5 and 10 µM inhibited Cyclin D1 protein levels 28% and 64% respectively; FH535 at 5 and 10 µM inhibited Survivin protein levels 24% and 48% respectively. The experiment was done twice with similar results. ER -