%0 Figure %A Gedaly, Roberto %A Galuppo, Roberto %A F. Daily, Michael %A Shah, Malay %A Maynard, Erin %A Chen, Changguo %A Zhang, Xiping %A A. Esser, Karyn %A A. Cohen, Donald %A Evers, B. Mark %A Jiang, Jieyun %A T. Spear, Brett %D 2014 %T FH535 inhibits proliferation of LCSC and HCC cell lines. %U https://plos.figshare.com/articles/figure/_FH535_inhibits_proliferation_of_LCSC_and_HCC_cell_lines_/1061191 %R 10.1371/journal.pone.0099272.g003 %2 https://ndownloader.figshare.com/files/1539268 %K cell biology %K Signal transduction %K cell signaling %K Signaling cascades %K Wnt signaling cascade %K Beta-catenin signaling %K Molecular cell biology %K genetics %K gene expression %K Gastroenterology and hepatology %K Liver diseases %K Hepatocellular carcinoma %K oncology %K Cancers and neoplasms %K Gastrointestinal tumors %K inhibits %K proliferation %K lcsc %K hcc %X

Cells were seeded in 96-well plates in 0.2 ml of media as described below for 72 hours, followed by the addition of 3H-thymidine at 1 µCi/well for 4 hours. Incorporation of 3H-thymidine was determined by scintillation counting. In panels A, B and D, the final concentration of DMSO in each well was 0.05%; in panel C, the final DMSO concentration in each well was 0.1%. (A) LCSCs were plated at 1000 cells/well in DMEM with 10% FBS along with DMSO alone or with increasing amounts of FH535. (B). LCSCs were plated at 5000 cells/well in DMEM with 1% FBS with DMSO alone or with increasing concentrations of FH535. (C). LCSCs were plated in DMEM with 10% FBS at 1000 cells/well with DMSO alone or increasing concentrations of XAV939. (D). Huh7, Hep3B and PLC cells were plated in DMEM with 10% FBS at 1000, 2500, and 5000 cells/well, respectively, with DMSO alone or increasing concentrations of FH535. P values are for all the three cell lines treated with FH535 are compared to controls. The experiment was done twice with similar results.

%I PLOS ONE