TY - DATA T1 - BmSpry was essential for antiviral defense in vivo. PY - 2014/06/10 AU - Shengkai Jin AU - Tingcai Cheng AU - Liang Jiang AU - Ping Lin AU - Qiong Yang AU - Yang Xiao AU - Takahiro Kusakabe AU - Qingyou Xia UR - https://plos.figshare.com/articles/figure/_BmSpry_was_essential_for_antiviral_defense_in_vivo_/1052722 DO - 10.1371/journal.pone.0099200.g005 L4 - https://ndownloader.figshare.com/files/1529266 KW - cell biology KW - Signal transduction KW - cell signaling KW - Molecular cell biology KW - immunology KW - Clinical immunology KW - immunopathology KW - microbiology KW - Virology KW - Viral replication KW - Virus effects on host gene expression KW - antiviral N2 - (A) The silkworm Nm DZ line was used for the RNAi experiment. Injection of dsRNA is indicated, the dsRNA of dsRed was used as a negative control with dsRNA of BmSpry as the experimental group. At 3 days post-injection of dsRNA, total RNA was extracted for qPCR to measure the expression level of BmSpry. Data are given as mean ±SD (n = 3). (B) Nm DZ silkworms treated with dsRNA against the indicated genes were infected with BmNPV (106 pfu/mL) by stab inoculation for 3 days and processed for qPCR. Data are given as mean ±SD (n = 3). (C) Analysis of the expression level of BmSpry in Nm DZ and the mutant DZ SN. Total RNA extracted from newly exuviated Nm DZ and DZ SN 5th instar larvae was used for qPCR. Data are given as mean ±SD (n = 3). (D) Analysis of mortality of DZ SN and Nm DZ after oral inoculation with BmNPV. Nm DZ and DZ SN newly exuviated 4th instar larvae were used for this experiment. DZ SN and Nm DZ silkworms were infected with BmNPV per os using a dose of 104 or 106 OB/larva and mortality was monitored until the adult stage (22 days). (E) Newly exuviated 2nd-instar larvae were used to investigate mortality after inoculation per os with 5×103 OB/larva. Mortality was monitored until the pupa stage (16 days). A representative of triplicate experiments is shown. Statistically significant differences: ** P<0.01. ER -