BmSpry regulation of ERK activation inhibited BmNPV replication. Shengkai Jin Tingcai Cheng Liang Jiang Ping Lin Qiong Yang Yang Xiao Takahiro Kusakabe Qingyou Xia 10.1371/journal.pone.0099200.g004 https://plos.figshare.com/articles/figure/_BmSpry_regulation_of_ERK_activation_inhibited_BmNPV_replication_/1052721 <p>(A) Firstly, BmE cells were transfected. The empty vector 1180 was used as a negative control and the BmSpry-oe vector was used as the experimental group with over-expression of <i>BmSpry</i>. At 2 days post-transfection, BmE cells were infected with BmNPV at MOI of 10. Post infection for 24 h, total proteins were extracted for western blotting using anti-phospho-ERK antibody. Total protein levels were measured with anti-GAPDH antibody. (B) BmN4-SID1 cells were used. After RNAi of 5 days, the BmN4-SID1 cells were infected with BmNPV at MOI of 10. Post infection for 24 h, total proteins were extracted for western blotting. A representative of triplicate experiments is shown.</p> 2014-06-10 03:29:23 cell biology Signal transduction cell signaling Molecular cell biology immunology Clinical immunology immunopathology microbiology Virology Viral replication Virus effects on host gene expression erk activation inhibited bmnpv