10.1371/journal.pone.0097359.g009 Chunfan Jiang Chunfan Jiang Tao Huang Tao Huang Yun Wang Yun Wang Guowei Huang Guowei Huang Xia Wan Xia Wan Jiang Gu Jiang Gu IgG expression was strongly correlated to MTA1 expression and IgG down-regulation lowered MTA1 expression. Public Library of Science 2014 Biochemistry proteins Immune system proteins antibodies cell biology Cell processes Cell growth Molecular cell biology genetics gene expression immunology Clinical immunology immunopathology oncology Basic cancer research metastasis Cancers and neoplasms carcinomas adenocarcinomas Adenocarcinoma of the lung Squamous cell carcinomas Squamous cell lung carcinoma Lung and intrathoracic tumors Cancer detection and diagnosis Pathology and laboratory medicine Clinical pathology correlated mta1 igg down-regulation lowered 2014-05-22 03:31:30 Figure https://plos.figshare.com/articles/figure/_IgG_expression_was_strongly_correlated_to_MTA1_expression_and_IgG_down_regulation_lowered_MTA1_expression_/1034681 <p><b>A</b>, IgG was down-regulated with specific IGHG1 siRNA transfection with Real-time PCR. <b>B</b>, MTA1 expression was lowered following the decrease of IgG expression detected with Real-time PCR. <b>C</b>, typical bands represent the changes of IgG and MTA1 expressions after IGHG1 siRNA interference in A549 cell line. <b>D</b>, similar changes in SK-MES-1 cell line. <b>E</b>, Igγ (red, positive staining in cytoplasm) was mostly co-localized with MTA1 (purplish red, positive staining in nuclei) in lung cancers. Black arrows or arrowheads show typical cancer cells labeling with IgG and MTA1. <b>F</b>, lung cancers with weak IgG expression have a lower expression level of MTA1 in comparison to strong IgG expression cancers. <b>G</b>, lung cancers with lymph node metastasis have much higher MTA1 expression than non-metastatic cancers. The histograms represent the four independent assays. Data are presented as mean ± S.E. *, <i>p</i><0.05. Scale bars: 20 µm.</p>