Watanabe, Etsuko Mano, Shoji Nishimura, Mikio Yamada, Kenji Phenotypic analysis of RNAiUBL5b in root elongation and development. <p>(A) Growth of RNAiUBL5b#1, RNAiUBL5b#2, and WT. Plants were grown for 7 days. Bar = 5 mm. (B) Root lengths of RNAiUBL5b#1 and RNAiUBL5b#2 plants. (C) Relative expression levels of <i>AtUBL5a</i> and <i>AtUBL5b</i> in RNAiUBL5b#1 and RNAiUBL5b#2 plants were estimated by quantitative real time polymerase chain reaction (qRT-PCR) and compared to that of WT plants. The expression level of each gene was normalized to that of ACTIN 2 (<i>ACT2</i>). (D) Auxin sensitivity of RNAiUBL5b#1. RNAiUBL5b#1 and control WT seedlings were cultivated for 5 days, then transferred to MS media containing different concentrations of 2,4-D (auxin). Bar = 10 mm. (E) The sensitivity of RNAiUBL5b#1 and WT to auxin was assessed by measuring root elongation defects and the number of lateral roots. Values in (B), (C), and (E) represent mean ± standard deviations of 8–10 samples of three independent experiments. Asterisks in (B), (C), and (E) indicate statistical significance according to Student’s t test ***<i>P</i> < 0.000 1, **<i>P</i> < 0.001.</p> HIND;Arabidopsis thaliana Ubiquitin-like proteins;ubiquitin-like modifier;AtPRP 38C;IAA 1;Hub 1 interaction domain;Hub 1-mediated pre-mRNA;plant root elongation;AtUBL 5a;AtUBL 5b;Snu 66 non-covalently;Hub 1;Yeast two-hybrid analysis;factor AtPRP 38;AtUBL 5;Hub 1 homologues;UBL 5 gene expression 2019-11-15
    https://plos.figshare.com/articles/figure/Phenotypic_analysis_of_RNAiUBL5b_in_root_elongation_and_development_/10313378
10.1371/journal.pone.0224795.g002