10.1371/journal.pone.0096250.g002 Linda Ravenna Linda Ravenna Lorenzo Principessa Lorenzo Principessa Alessandra Verdina Alessandra Verdina Luisa Salvatori Luisa Salvatori Matteo Antonio Russo Matteo Antonio Russo Elisa Petrangeli Elisa Petrangeli Hypoxic regulation of NF-kB nuclear expression in LNCaP, DU145 and PC3 cells. Public Library of Science 2014 Biochemistry Oxidative damage cell biology Cell processes Cellular stress responses Molecular cell biology genetics gene expression DNA transcription Protein translation immunology Immune response inflammation Clinical immunology immunity oncology Cancers and neoplasms Genitourinary tract tumors Prostate cancer Basic cancer research nf-kb du145 pc3 2014-05-06 02:56:37 Figure https://plos.figshare.com/articles/figure/_Hypoxic_regulation_of_NF_kB_nuclear_expression_in_LNCaP_DU145_and_PC3_cells_/1017637 <p>Cell were exposed to 1% O<sub>2</sub> from 0.5 up to 24 h or left untreated. After the times indicated, cells were processed and the nuclear content of p50 and p65 was detected by immunoblot analysis. β-actin was used as a loading control. A representative experiment for each gene in all the studied cell lines is shown. Mean densitometry of NF-kB p50 and p65 relative to β-actin ± SE is also evidenced and expressed as arbitrary units. * Hypoxic cells <i>vs</i> normoxic control cells.</p>