TY - DATA T1 - Time-course of hypoxia on HIF1α, HIF2α and HIF3α protein nuclear level and mRNA expression in LNCaP, DU145 and PC3 cells. PY - 2014/05/06 AU - Linda Ravenna AU - Lorenzo Principessa AU - Alessandra Verdina AU - Luisa Salvatori AU - Matteo Antonio Russo AU - Elisa Petrangeli UR - https://plos.figshare.com/articles/figure/_Time_course_of_hypoxia_on_HIF1_945_HIF2_945_and_HIF3_945_protein_nuclear_level_and_mRNA_expression_in_LNCaP_DU145_and_PC3_cells_/1017636 DO - 10.1371/journal.pone.0096250.g001 L4 - https://ndownloader.figshare.com/files/1486466 KW - Biochemistry KW - Oxidative damage KW - cell biology KW - Cell processes KW - Cellular stress responses KW - Molecular cell biology KW - genetics KW - gene expression KW - DNA transcription KW - Protein translation KW - immunology KW - Immune response KW - inflammation KW - Clinical immunology KW - immunity KW - oncology KW - Cancers and neoplasms KW - Genitourinary tract tumors KW - Prostate cancer KW - Basic cancer research KW - hypoxia KW - mrna KW - du145 KW - pc3 N2 - Cell were exposed to 1% O2 from 1 up to 48–72 h, according to the experimental design or left untreated. A Protein expression was detected in nuclear extracts by immunoblot analysis. A representative experiment for each gene in each studied cell lines is shown. β-actin was used as a loading control. B mRNA determinations were performed by real-time PCR, normalized to the housekeeping gene 18S rRNA and expressed as fold induction with respect to normoxic controls (set at 1). Mean values ± SE. ER -